| 胰岛素对糖尿病大鼠视网膜VEGF和Ang-2表达的影响 |
| 投稿时间：2009-10-26 修订日期：2009-12-03 点此下载全文 |
| 引用本文：马萍萍、王玉粦、吴炎、李斌、林少芬、唐仕波.胰岛素对糖尿病大鼠视网膜VEGF和Ang-2表达的影响[J].医学研究杂志,2010,39(1):61-63 |
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| 基金项目:深圳市科技计划项目（200404048） |
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| 中文摘要:目的观察胰岛素治疗对糖尿病大鼠视网膜血管内皮细胞生长因子（vascular endothelial growth factor, VEGF）和血管生成素-2（angiopoietin-2, Ang-2）表达的影响，探讨胰岛素在糖尿病视网膜病变发生发展中的作用。方法链尿佐菌素（streptozotocin, STZ）诱导糖尿病大鼠动物模型，成模后经胰岛素治疗控制血糖，随机分为糖尿病血糖严格控制组、糖尿病血糖非严格控制组、糖尿病组和正常对照组。应用免疫荧光组织化学染色法检测大鼠视网膜组织VEGF、Ang-2的表达，用计算机-图像分析软件对VEGF、Ang-2免疫荧光强度进行半定量分析。结果与正常对照组比较，糖尿病组大鼠视网膜VEGF、Ang-2平均荧光强度分别增加了2.38倍和2.41倍（P<0.05）。与糖尿病组比较，血糖严格控制组大鼠视网膜VEGF、Ang-2的表达分别下降了1.47倍和1.51倍（P<0.05），而血糖非严格控制组VEGF、Ang-2的表达无明显下降（P>0.05）。结论胰岛素治疗严格控制血糖可降低糖尿病大鼠视网膜VEGF、Ang-2的表达，对糖尿病视网膜病变的发生发展有保护作用。 |
| 中文关键词:糖尿病视网膜病变、胰岛素、血管内皮细胞生长因子、血管生成素－2 |
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| The Effect of Insulin Therapy on Expression of VEGF and Ang-2 in Diabetic Rat Retina |
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| Abstract:ObjectiveTo observe the changes of VEGF and Ang-2 expression in streptozotocin(STZ)-induced diabetic rat retina after insulin therapy and explore possible roles of insulin in the development of diabetic retinopathy. MethodsDiabetes was induced in 8-week-old male wistar rats by a single intraperitoneal injection of STZ. After three weeks, animals were randomly divided into four groups:(1)diabetic rats received intensive insulin therapy for 20 days;(2)diabetic rats received unregular insulin therapy, which caused the abrupt fluctuation of glycemic level;(3)diabetic control rats; and (4)normal control rats. After treatment, the animals were sacrificed with an overdose of anesthesia,and the eyes were enucleated and fixed in 4% paraformaldehyde immediately. Paraffin sections of retina were prepared.Expression of VEGF and Ang-2 was assessed by immunofluorescence stain and images analysis. ResultsQuantitative analysis showed VEGF and Ang-2 protein expression was increased by 2.38-fold and 2.41-fold in diabetic rats retinas as compared to non-diabetic rats retinas respectively (P<0.05). The expression of VEGF and Ang-2 was decreased by 1.47-fold and 1.51-fold in diabetic rats which received intensive therapy respectively (P<0.05). And there were no significant decreases in VEGF and Ang-2 expression in diabetic rats which received unregular insulin therapy（P>0.05）. ConclusionIntensive insulin therapy could decrease VEGF and Ang-2 expression in retina and has protective effect on diabetic retinopathy in STZ-diabetic rats. |
| keywords:Diabetic retinopathy Insulin Vascular endothelial growth factor Angiopoientin-2 |
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