| 生长激素-海藻酸钠-壳聚糖微胶囊的制备及药物释放性能研究 |
| 投稿时间：2009-12-04 修订日期：2009-12-25 点此下载全文 |
| 引用本文：赛佳明,张亦军,黄勇,张增方,江晓路.生长激素-海藻酸钠-壳聚糖微胶囊的制备及药物释放性能研究[J].医学研究杂志,2010,39(3):88-93 |
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| 基金项目:青岛市2008年度医药科研指导计划（2008-wszd126) |
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| 中文摘要:摘要目的研究生长激素-海藻酸钠-壳聚糖微胶囊的制备及其释放性能。方法本课题采用脉冲电场法制备生长激素-海藻酸钠-壳聚糖微球。根据单因素试验和正交试验结果，优化工艺条件和处方组成。观测其形态、尺寸, 鉴别其组分, 测定生长激素含量、包封率和回收率, 并进行体内、外释放实验。结果选择450μm锐孔直径、2cm液面距、1.5%海藻酸钠浓度、8ml/h推进速度、2% Ca2+浓度作为最佳工艺条件。光学显微镜观察可见得到的微胶囊具有很好的圆形形态,平均粒径尺寸为47.93μm。微胶囊的平均包封率为94% , 他莫昔芬平均含量为11.24%。体外模拟胃液中，微球不溶胀而且所载生长激素基本不被释放；在模拟肠液中，微球溶胀而且所载生长激素被缓释出来,并且在12h后被完全释放出来。体内实验证实生长激素微球组的血药浓度在8h时到达峰值(98.59ng/ml)。体内、外释放证实能够达到延缓释放的目的。结论生长激素-海藻酸钠-壳聚糖微球具有良好的圆形形态和较好缓释效果。 |
| 中文关键词:生长激素 海藻酸钠 壳聚糖微胶囊 微胶囊 模拟胃、肠液 体内、外药物释放 |
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| Preparation of Growth Hormone-Chitosan-Alginate Microcapsules and the Release Profile |
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| Abstract:AbstractObjectiveTo study the preparation and the in vitro and in vivo release profile of GH-Chitosan-Alginate microcapsules.MethodsGH-Chitosan-Alginate microcapsules were prepared through impulsive electrostatic technique. The interrelated factors influencing the diameter and sphericity were studied through orthogonal experiments，and finally the statistic analysis made sure the optimum conditions to prepare microspheres. The morphology and size of the microcapsules were observed, and the content, encapsulation efficiency and recovery efficiency of the microcapsules were measured. Moreover, their in vitro and in vivo release experiments were carried out. ResultsThe results showed that the diameter of needle was the most significant factor to the diameter of microspheres. The optimum conditions for the least diameter of microspheres were 450μm diameter of needle，2cm from needle tips to the gelation surfaee，1.5% alginate concentration, 8ml/h speed of flowing-liquid and metal containers. The microcapsules had good sphericity morphology and distribution. The size of the microcapsules was in the range of 10-25μm with an average size of 47.93μm. The encapsulation efficiency and GH-load of the microcapsules were 94% and 11.24% respectively. The release kinetics of microcapsules was studied in false gastric and intestines juice. In false gastric juice, the GH of microcapsules was not released; in false intestines juice, it was released well, and TAM was completely released after about 12h. in vivo release profile made sure that the serum GH level of GH microcapsule group was at the highest value (98.59ng/ml) at 8h. The release profile was fitted well in both in vitro and in vivo conditions. ConclusionGH-Chitosan-Alginate Microcapsules have good morphology and sustained release effect. |
| keywords:Growth hormone(GH) Chitosan Alginate Microcapsule False gastric and intestines juice in vitro and in vivo release profile |
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