| 一种Alzheimer痴呆小鼠模型的建立 |
| 投稿时间：2009-12-08 修订日期：2009-12-16 点此下载全文 |
| 引用本文：王清华,徐如祥.一种Alzheimer痴呆小鼠模型的建立[J].医学研究杂志,2010,39(4):54-56 |
| DOI：
|
| 摘要点击次数: 1212 |
| 全文下载次数: 1052 |
|
|
| 中文摘要:目的建立Alzheimer痴呆（Alzheimer dementia, AD）小鼠模型。方法C57BL/6小鼠基底巨细胞核（NBM）注射Ibotenic 酸制成AD模型。8周后应用8方向迷宫评价小鼠痴呆程度，应用免疫组织化学染色观察NBM和脑皮质胆碱能系统和β-淀粉样蛋白表达改变。结果NBM内胆碱能神经元明显减少，5-羟色胺（5-HT）神经元和GABA神经元轻度减少。同侧额、顶叶皮质内胆碱能神经纤维显著减少，5-HT神经轴突轻度减少，伴大量β-淀粉样蛋白表达,GABA神经元则无明显变化。NBM毁损小鼠的工作记忆错误显著升高，小鼠的近事记忆明显损害。结论NBM注射Ibotenic 酸能建立一种可靠的AD小鼠模型。 |
| 中文关键词:基底巨细胞核 胆碱能神经元 Alzheimer痴呆模型 小鼠 |
| |
| Establishment of a Mouse Model of Alzheimer Dementia |
|
|
| Abstract:AbstractObjectiveTo establish a Alzheimer dementia(AD) model in mice. MethodsThe C57BL/6 mice were lesioned with ibotenic acid in Nucleus basalis of Meynert(NBM). Behavioral tests by eight-arm radial maze were conducted 8 weeks, and immunohistochemical staining of choline acetyltransferase(ChAT), serotonin（5-HT）, GAD（GABA）, amyloid-βprotein (AP) was conducted 12 weeks after NBM lesioning. ResultsIn NBM lesioned mice, the ChAT-positive neurons, serotonin-positive neurons, and GAD-positive neurons in right NBM reduced, and ChAT-positive neurons reduced most evidently. At the same time, the ChAT-positive fibers in prefrontal and parietal cortices decreased significantly, serotonin-positive axons slightly, accompanied by heavily AP co-expression. On the contrary, there was no change of GAD-positive neurons in cortex. The working memory error increased significantly.ConclusionIbotenic acid lesioning in NBM can provide as a model of AD in that it produces deafferentation of cholinergic system and recent memory disruption. |
| keywords:Nucleus basalis of meynert Cholinergic neuron Alzheimer dementia model Mouse |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
