| 芪苈强心胶囊治疗慢性心力衰竭大鼠的实验研究 |
| 投稿时间：2009-12-24 点此下载全文 |
| 引用本文：林锐波,朱剑荣,王伟,傅玉才.芪苈强心胶囊治疗慢性心力衰竭大鼠的实验研究[J].医学研究杂志,2010,39(4):62-66 |
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| 中文摘要:目的观察芪苈强心胶囊对慢性心力衰竭大鼠心功能及凋亡蛋白caspase-3的影响，探讨治疗慢性心力衰竭的作用机制。方法结扎大鼠冠状动脉左前降支，建立AMI模型，存活者随机分为模型组、芪苈强心胶囊高、中、低剂量组(简称高、中、低剂量组)和卡托普利组，另设假手术组。芪苈强心胶囊组术后予药粉1.2g/（kg·d）、0.6g/（kg·d）、0.3g/（kg·d）治疗，卡托普利组予卡托普利25mg/（kg·d）治疗，模型组和假手术组仅予等体积生理盐水，治疗4周后对相关指标进行检测。结果与模型组相比，芪苈强心胶囊能有效降低各治疗组体重(BW)、心脏质量指数(HMI)、左心室质量指数（LVMI）及左心室舒张末压（LVEDP），有效增加左心室收缩末压（LVSP）左心室最大压力上升及下降速度(±dp/dtmax)，以高剂量组较为明显；模型组caspase-3蛋白表达较假手术组显著增加，芪苈强心胶囊治疗高、中剂量组较模型组显著降低。结论芪苈强心胶囊治疗能明显改善AMI心力衰竭大鼠心功能，下调caspase-3蛋白表达水平，有效抑制心力衰竭后心肌细胞凋亡。推测芪苈强心胶囊能有效改善心功能的作用机制可能与其抑制心肌重构及心肌细胞凋亡有关。 |
| 中文关键词:芪苈强心胶囊 心肌梗死 心力衰竭 凋亡 caspase-3 |
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| The Effect of Qiliqiangxin on Chronic Heart Failure Rat Model |
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| Abstract:AbstractObjectiveTo evalute Qiliqiangxin capsule for improving cardiac function and identifiy Qiliqiangxin capsule for directing cytoprotective effects against apoptosis of cardiomyocytes. MethodsSprague Dawley (SD) rats were tested as subjects and the model rats were built by anterior descending coronary deligation, then the survival rats were devided into 6 groups randomly:sham-operated group（sham，n=8）,fed with normal saline of same volume instead；control group（control，n=7）,fed with normal saline of same volume instead；Qiliqiangxin great dosage group（great，n=9）,Qiliqiangxin 1.2g/（kg·d） treatment；Qiliqiangxin medium dosage group（medium，n=9）,Qiliqiangxin 0.6g/（kg·d） treatment；Qiliqiangxin small dosage group（small，n=8 rats）,Qiliqiangxin 0.3g/（kg·d） treatment；the captopril group（captopril，n=9）,captopril 25mg/（kg·d） treatment.24 hours later,rats in sham operated group and model group were given normal saline,rats in treating group were given Qiliqiangxin,rats in the contrasting group were given captopril.After being treated for 4 weeks, all rats′ heart function was measruated by right flank arteria carotis communis arterial cannula.The left ventricle mass index was calculated and the expression of protein caspase-3 was tested by immunohistochemical method and Westernblot. Results Compared with control group, Qiliqiangxin capsule could reduce effectively BW,HMI,LVMI，increase LVEDP and LVSP,+dp/dtmax and -dp/dtmax.Immunohistochemical analysis and Westernblot analysis showed that adminstration of Qiliqiangxin significantly inhibited caspase-3 expressions in myocardial cells in a dose-dependent manner.ConclusionQiliqiangxin significantly improved the CHF rat′s cardiac function and reduced the left ventricle mass index and heart mass index 4 weeks after being treated with relative high dose Qiliqiangxin. It might be partially attributed to the suppression of caspase-3 expressions. |
| keywords:Qiliqiangxin Chronic heart failure AMI caspase-3 |
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