| 纳洛酮对大鼠心肌缺血再灌注损伤的影响 |
| 投稿时间：2009-11-01 修订日期：2009-12-01 点此下载全文 |
| 引用本文：高致炳,王云,顾继伟,冯东,刘超,袁亚伟.纳洛酮对大鼠心肌缺血再灌注损伤的影响[J].医学研究杂志,2010,39(4):90-94 |
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| 中文摘要:目的观察纳洛酮(naloxone, NAL)对大鼠心肌缺血再灌注损伤的影响，并探讨其机制。 方法36只雄性SD大鼠随机分为空白对照组(Con组) 、缺血再灌注组（I/R组）、缺血预处理组（IPC组）和纳洛酮预处理组(Nal+I/R组)，观察各组在缺血再灌注后的血流动力学变化、心肌梗死面积和心律失常发生情况，光镜下观察各组心肌超微结构的改变，用TUNEL检测法检测各组细胞凋亡情况。 结果与IR组相比，IPC组和Nal+I/R组均能明显改善缺血再灌注后心功能(P<0.05)，减少心肌梗死面积(P<0.01)及心律失常的发生(P<0.01)，保护心肌超微结构，减少凋亡指数(P<0.01)。 结论纳洛酮对在体大鼠心肌缺血再灌注损伤心脏有保护作用，该研究拓宽了纳洛酮的临床用途，为防治缺血性心脏病提供了理论依据。 |
| 中文关键词:纳洛酮 缺血再灌注损伤 |
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| Effects of Naloxone on Myocardial Ischemia-reperfusion Injury in Rats |
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| Abstract:AbstractObjectiveTo observe the effect of naloxone(Naloxone,NAL) on myocardial ischemia-reperfusion injury in rats,and to explore its mechanism.Methods36 male SD rats were randomly divided into blank control group (Con group),ischemia-reperfusion group (I/R group),ischemic preconditioning group (IPC group) and naloxone pretreatment group (Nal+I/R group).Changes of cardiac function,myocardial infarct size and occurrence of arrhythmias after ischemia-reperfusion in each roup were observed.Changes of myocardium under light microscope ultrastructural in each group were also observed.Apoptosis situation in each group was detected by TUNEL assay.ResultsCompared with th IR group,IPC group and Nal+I/R group showed significantly improvement in cardiac function after ischemia-reperfusion (P＜0.05),reduce in myocardial infarct size(P＜0.01), and arrhytmia (P＜0.01),protection effect on myocardial ultrastructure,and reduce in apoptosis index (P＜0.01).ConclusionNaloxone had protective effect on myocardial ischemia-peperfusion injury in vivo in rat heart.The study broadened the clinical use of naloxone and provided a theoretical basis for the prevention and treatment of ischemic heart disease. |
| keywords:Noaloxone Ischemia-reperfusion injury Rat |
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