| TLR4对脑缺血再灌注小鼠海马IRF-3和IFN-β表达的影响 |
| 投稿时间：2009-12-30 点此下载全文 |
| 引用本文：陈燕飞,周丽,高音.TLR4对脑缺血再灌注小鼠海马IRF-3和IFN-β表达的影响[J].医学研究杂志,2010,39(5):58-60 |
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| 基金项目:黑龙江省自然科学基金资助项目（D2007-93） |
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| 中文摘要:目的探讨Toll样受体4（TLR4）对脑缺血再灌注小鼠海马IRF-3和IFN-β表达的影响。方法采用TLR4抗体封闭阻断TLR4，Western blot检测海马TLR4、IRF-3和IFN-β表达变化。小鼠随机分为3组：假手术组（S组）、缺血再灌注组（I组）、TLR4阻断组（T组），各组又分1天、2天、3天、4天 4个时间点组。结果缺血再灌注组TLR4、IRF-3和IFN-β表达水平明显高于假手术组表达水平（P<0.05），而TLR4阻断组表达水平明显少于缺血再灌注组（P<0.05）。结论缺血再灌注可激活TLR4，并引起IRF-3和IFN-β表达量增加，而采用TLR4抗体封闭阻断TLR4后，IRF-3和IFN-β表达量减少。提示TLR4通过上调IRF-3和IFN-β表达参与脑缺血再灌注的反应机制。 |
| 中文关键词:脑缺血再灌注 TLR4 TLR4阻断 IRF-3 IFN-β |
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| The Effect of Toll-like Receptor 4（TLR4）on Expression of IRF-3 and IFN-β During Cerebral Ischemia Reperfusion in Hippocampus in Mice |
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| Abstract:ObjectiveTo study the effect of Toll-like receptor 4（TLR4）on expression of IRF-3 and IFN-β during the inflammatory reaction induced by cerebral ischemia reperfusion in mice. MethodsAfter blockade of TLR4 by TLR4 antibody，expression of TLR4，IRF-3 and IFN-β at the protein level in hippocampus was examined by Western blot，respectively. Mice were randomly divided into sham group，ischemia reperfusion group and TLR4 blocking group in different time points （1，2，3 and 4 day）. ResultsIn the right cortex，the expression of TLR4, IRF-3 and IFN-β of I group was distinctly higher than that of S and T group（P<0.05）. Conclusion Our results indicate that TLR4 is activated by cerebral ischemia reperfusion in hippocampus in mice，while expression of IRF-3 and IFN-β was decreased after TLR4 was blocked by TLR4 antibody which means that TLR4 participates in cerebral ischemia reperfusion through upregulating expression of IRF-3 and IFN-β. |
| keywords:Cerebral ischemia reperfusion TLR4 TLR4-blocked IRF-3 IFN-β |
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