| CIK细胞对人肺癌细胞A549凋亡作用的影响 |
| 投稿时间：2009-12-25 修订日期：2010-03-12 点此下载全文 |
| 引用本文：师岩,张春晶,刘秀财,吴琦,髙涵,冯丽,王淑英,李淑艳.CIK细胞对人肺癌细胞A549凋亡作用的影响[J].医学研究杂志,2010,39(5):61-63 |
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| 基金项目:黑龙江省教育厅科研基金项目 （11541406） |
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| 中文摘要:目的观察细胞因子诱导的杀伤细胞（cytokine induced killer cell ,CIK）对人肺癌细胞A549凋亡作用的影响，并探讨机制。方法常规方法体外诱导生成CIK细胞, CIK细胞为效应细胞,人肺癌细胞A549为靶细胞，比较观察人肺癌细胞A549的凋亡情况。 结果电镜法观察CIK细胞能诱导人肺癌细胞A549出现凋亡的超微结构变化，annexinV/PI流式细胞分析法检测早期凋亡率发现CIK实验组明显高于对照组。结论体外培养的CIK细胞可明显诱导人肺癌细胞A549凋亡。 |
| 中文关键词:CIK细胞 人肺癌细胞A549 细胞凋亡 |
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| he Effect of CIK Cell on Apoptosis of Lung Cancer Cell A549 |
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| Abstract:ObjectiveTo investigate the inhibitory effects of CIK cell on apoptosis of lung cancer cell A549 in vivo. MethodsCIK cells were induced by culturing PBMC with regular method and cell apoptosis was detected.ResultsElectron microscopic observations showed that CIK cells could induce the lung cancer cells A549 to apoptosis. Flow cytometry（FCM）demonstrated that apoptosis cells of lung cancer cells A549 were increased in CIK group as compared with the control group. ConclutionCIK cells can induce the apoptosis of lung cancer cells A549. |
| keywords:Cytokine induced killer cell Lung cancer cell A549 Apoptosis |
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