| 快速老化小鼠SAMP8海马组织中基因表达谱的研究 |
| 投稿时间：2010-03-30 点此下载全文 |
| 引用本文：戴大鹏,宋晓宁,郑君德,蔡剑平.快速老化小鼠SAMP8海马组织中基因表达谱的研究[J].医学研究杂志,2010,39(6):23-26 |
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| 基金项目:科技部国际科技合作计划（2006DFB31410），卫生部北京医院面上项目（BJ-2008-77） |
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| 中文摘要:目的观察快速老化小鼠P8（SAMP8）与同龄正常老化小鼠R1（SAMR1）海马组织中基因表达谱的差异。方法选用1、4、6、8、12月龄的SAMP8小鼠，用SAMR1作为对照组，每组各9只，快速处死后取海马组织提取总RNA, 用纯化后的总RNA合成cDNA并标记荧光染料，与32k小鼠寡核苷酸微阵列芯片杂交，用共聚焦扫描仪LuxScanTM 10KA及LuxScan 3.0软件进行图像采集与数据处理，用SAM软件分析差异表达的基因，用MAS系统对差异基因进行通路（pathway）分析。结果正常老化小鼠SAMR1各月龄间表达谱差异无显著性，快速老化小鼠SAMP8各月龄间差异显著，两种品系小鼠的比较结果显示1月龄、12月龄基因表达差异最为显著，6月龄无显著差异。Pathway分析结果显示MAPK信号通路的基因表达差异最为显著。结论SAMP8小鼠与SAMR1小鼠海马的基因表达存在明显的差异，以MAPK信号通路的基因表达差异最为显著。 |
| 中文关键词:快速老化小鼠 基因芯片 pathway分析 |
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| Gene Expression Profiles in the Hippocampus of Senescence-accelerated Mouse SAMP8 |
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| Abstract:ObjectiveTo explore the different expression patterns of senescence accelerated mouse substrain SAMP8 and control substrain SAMR1 in different month ages. MethodsNine SAMP8 and SAMR1 mice at 1, 4, 6, 8, 12 month age were sacrificed and 3 hippocampus of each set were pooled as one starting samples for RNA extraction. After being purified, some total RNAs were reverse transcripted and labeled for oligonucleotide microarray analysis with approximately 32 000 probes. After hybridization, slides were scanned with a confocal scanner LuxScanTM 10KA and the data were extracted from images using LuxScan 3.0 software. Differentially expressed genes were identified using SAM software and pathway analysis was performed using MAS platform which was constructed by CapitalBio Cor. ResultsWithin each substrain, unsupervised clustering analysis revealed that SAMP8 mice showed different expression patterns in different month ages whereas SAMR1 mice were not. On the another hand, at the same month age, SAMP8 and SAMR1 mice at 1 month and 12 month had largest differently expressed genes. Interestingly, mice at 6 month could not be detected having statistically differently expressed genes. Pathway analysis showed that most of these statistically expressed genes were involved in the MAPK signaling pathway. ConclusionExpression patterns of SAMP8 and SAMR1 were significantly different in different month ages and the genes involved in MAPK signaling pathway had the largest numbers in all statistically expressed genes. |
| keywords:Senescence-accelerated mouse Gene chip Pathway analysis |
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