| 混合交联剂制备海藻酸盐微囊及其性能研究 |
| 投稿时间：2010-01-12 修订日期：2010-04-07 点此下载全文 |
| 引用本文：童粤生,沈德勇,王士斌,白燕,陈爱政.混合交联剂制备海藻酸盐微囊及其性能研究[J].医学研究杂志,2010,39(6):27-31 |
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| 基金项目:国家863 计划（2006AA02A118）；国家自然科学基金资助项目(30370415)；福建省自然科学基金（C0710034、2009J01253）资助项目。 |
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| 中文摘要:目的以氯化钙和氯化钡混合盐溶液为交联剂，制备海藻酸盐微囊并考察其性能。方法采用高压静电法制备胶珠，以几丁聚糖为膜材制备微胶囊，考察了海藻酸盐胶珠和微囊的形态、粒径分布、膨胀率、振荡破损率及膜蛋白渗透性。结果以［Ba2+］∶［Ca2+］为7∶3作为混合交联剂制备的微囊粒径最小为309μm，膨胀率最小为147%，振荡破损率为26%。各组微囊膜厚度均小于20μm。两种蛋白渗透曲线表明，胰蛋白酶能渗透进入各组微囊膜，而牛血清清蛋白则不能进入微囊。结论混合交联剂制备的微囊粒径分布较均一，膜强度较高，可广泛应用于各种生物活性物质如蛋白、细胞等的包埋。 |
| 中文关键词:微胶囊 混合并联剂 膨胀率 膜厚度 蛋白通透性 |
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| Preparation and Properties Evaluation of Alginate-chitosan Microcapsules Cross-linked by Mixed Metal Irons |
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| Abstract:ObjectiveThe alginate-chitosan microcapsules were prepared,and mixed metal irons (Ba2++Ca2+) were selected as cross linkers.MethodsMicrocapsules were prepared by electrostatic combination on the high-voltage electrostatic droplet generator,and their size distribution,film thickness,breakage rate and protein permeability were compared.ResultsWe found that when the ration of ［Ba2+］∶［Ca2+］ was 7∶3,the microcapsules showed the maximum value of average sized(309μm) and the minimum number of dilatability (14.7%).The film thickness of all five kinds of microcapsules was lower than 20μm.Results of trypsinase and BSA permeability showed that the transmission percent of trypsinase (24kDa) was nearly 50% and BSA could be intercepted by five different microcapsules.ConclusionMicrocapsules prepared by mixed metal irons (Ba2++Ca2+) cross linkers had uniform size and high intensity,and could be widely applied for embedding various bio-active substances such as proteins and cells. |
| keywords:Microcapsules Mixed cross linkers Dilatability Breakage rate Protein permeability |
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