| 贝那普利对年轻自发性高血压大鼠左室心肌胶原的影响 |
| 投稿时间：2010-01-20 点此下载全文 |
| 引用本文：孙绍贤,李海涛,刁增利,唐蕊,赵红,宋文静.贝那普利对年轻自发性高血压大鼠左室心肌胶原的影响[J].医学研究杂志,2010,39(6):63-66 |
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| 基金项目:河北省教育厅资助项目（2004120）;河北省卫生厅资助项目（04263）;华北煤炭医学院附属医院资助项目（08009） |
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| 中文摘要:目的探讨贝那普利对年轻自发性高血压大鼠左室心肌间质及血管周围胶原影响及其机制。方法16只自发性高血压大鼠（SHR）投币法随机分为模型组(n=8)、贝那普利治疗组(n=8)。7只WKY大鼠为正常血压大鼠组。治疗组给予贝那普利［10mg/(kg·d）］灌胃，1次/日，其余两组蒸馏水灌胃。治疗12周后，颈动脉插管，测颈动脉收缩压（SBP）和舒张压(DBP)；血清及左室心肌组织血管紧张素Ⅱ浓度(AngⅡ)，应用苦味酸-天狼猩红染色法，计算机图像分析软件分析左室心肌组织切片胶原容积分数(CVF)和心肌小动脉周围胶原面积与小动脉管腔面积比(PVCA/LA)，观察血管周围胶原；用偏光显微镜观察并分析Ⅰ型胶原、Ⅲ胶原，计算Ⅰ/Ⅲ型胶原比值，观察左室心肌间质胶原。结果治疗12周后，模型组颈动脉SBP、DBP、左室AngⅡ、CVF、PVCA/LA及Ⅰ/Ⅲ型胶原比值均显著高于正常血压大鼠（WKY）对照组（P<0.01）；而贝那普利治疗组各指标与模型组比较，均显著性下降（P<0.01)，有效降低SHR的SBP、DBP，CVF、PVCA、Ⅰ/Ⅲ型胶原，降低心肌局部AngⅡ表达，可预防SHR心肌纤维化。结论早期应用贝那普利治疗降低SHR心肌胶原，可预防心肌纤维化的形成，机制可能与降低心肌局部AngⅡ表达有关。 |
| 中文关键词:贝那普利 年轻自发性高血压大鼠 血管紧张素Ⅱ 心肌纤维化 |
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| The Effect of Benazepril on Left Ventriclar Myocardial Interstitial Fibrosis in Early Spontaneous Hypertensive Rats |
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| Abstract:ObjectiveTo study the effect of benazepril on left ventriclar myocardial fibrosis and mechanism in the early spontaneously hypertensive rats. MethodsNormal Wistar-Kyoto (WKY,n=7) rats were used as control，and spontaneously hypertensive rats (SHR) were randomly to receive placebo (n=8) or benazepril tablets (n=8)．Benazepril ［10mg/（kg·d）,qd］ was given to rats for 12 weeks.Systolic blood pressure(SBP) and diastolic blood pressure(DBP) of the carotid artery were detected. Angiotensin Ⅱ of blood-serum and left ventricular myocardium tissuse were determined.Picrosirius red staining combined with computed myocardial fibrosis were employed to evaluate the collagen volume fraction (CVF),and perivasular collagen area/luminal area ratio (PVCA/LA)．Type Ⅰ and Ⅲ collage were measured with polarimicroscope,then Ⅰ/Ⅲ type collagen rate was calculated.ResultsCompared with WKY group,SBP,DBP,CVF,PCVA/LA and Ⅰ/Ⅲ type collagen rate all increased markedly in the SHR group.And Benazepril groups showed obrious reduction in muscle AngⅡ (P<0.01),CVF(P<0.01), PCVA/LA (P<0.01) and Ⅰ/Ⅲ type collagen rate (P<0.01),and SBP (P<0.01) DBP(P<0.01) after 12 weeks.ConclusionChronic administration of Benazepril could inhibit and prevent the development of myocardial fibrosis in the early spontaneously hypertensive rats．Decreasing AngⅡ of myocardium tissuse may be involved in the inhibition mechanism of myocardial fibrosis by Benazepril Tablets． |
| keywords:Benazepril Tablets Early spontaneously hypertensive rats Angiotensin Ⅱ Myocardial interstitial fibrosis |
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