| 胃癌细胞P53、P16蛋白表达与化疗药物敏感性的关系 |
| 投稿时间：2010-01-26 修订日期：2010-04-05 点此下载全文 |
| 引用本文：耿明,王琳,刘晓红,曹永成,景洪标.胃癌细胞P53、P16蛋白表达与化疗药物敏感性的关系[J].医学研究杂志,2010,39(6):79-81 |
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| 基金项目:济南军区“十一五”计划课题（J115Z004） |
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| 中文摘要:目的探讨胃癌对化疗药物的耐药性及其与P53和P16蛋白表达的关系。方法收集75例新鲜胃癌标本,制备单细胞悬液，分别加入HCPT、CDDP、ADM、5-FU和MMC培养48h，用MTT比色法检测胃癌细胞对化疗药物的敏感性；免疫组化技术检测P53和P16 蛋白在胃癌组织中的表达。结果胃癌细胞对不同化疗药物敏感性不同：5-FU(43.4±9.2)、CDDP(41.9±8.7)和HCPT(40.6±8.3)对胃癌细胞的抑制率与ADM(31.6±7.8)和MMC(28.7±7.3)比较有统计学意义（P<0.05）。胃癌组织中P53和P16 蛋白的阳性率分别为81.33%(61/75)和38.67%(32/75)。P53阳性者显示对5-FU、ADM、HCPT有明显的体外耐药性（P<0.05），P16在5-FU和CDDP耐药组阳性率低于敏感组（P<0.05）。结论P53和P16蛋白参与了胃癌对化疗药物原发性耐药的产生。MTT药敏检测有助于筛选有效化疗药物。 |
| 中文关键词:胃肿瘤 化疗药物 敏感性 MTT比色 |
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| Relationship between Expression of P53，P16 and Chemosensitivity in Gastric Carcinoma |
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| Abstract:ObjectiveTo investgate the relationship between expression of P53,P16 and sensitivity to chemotherapeutic-drugs in gastric carcinoma. Methods75 samples of gastric cancer obtained from operating room were prepared to single-cell suspension and separately exposed to HCPT, CDDP, 5-Fu, ADM, MMC for 48 hours. The sensitivity of gastric cancer cells to chemotherapeutic drugs was evaluated with MTT assay.Expression of P53 and P16 was detected by immunohistochemistry. ResultsThe sensitivity of gastric carcinoma cells to chemotherapeutic drugs was different.Inhibitory rate of tumor cells exposed to 5-FU(43.4±9.2)，CDDP(41.9±8.7)and HCPT(40.6±8.3) were significantly higher than those of cells exposed to ADM(31.6±7.8) and MMC(28.7±7.3)（P<0.05）.Positive rate of P53 and P16 in gastric cancer was 81.33% and 38.67% respectively. P53 positive cells showed resistance to 5-FU，ADM and HCPT.P16 expression was significantly lower in the resistance to 5-FU and CDDP than that in the the sensitivity. Conclusion P53 and P16 may contribute to primary drug-resistance of gastric cancer. MTT chemosensitivity test could constitute the prediction on applying chemo-drugs and identify drug-resisting cases. |
| keywords:Gastric neoplasms Chemotherapeutic-drugs Sensitivity MTT colorimetric assay |
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