| 维生素C对非洲爪蟾卵母细胞ATP-激活电流的增强作用 |
| 投稿时间：2010-02-20 修订日期：2010-04-20 点此下载全文 |
| 引用本文：聂永莉,张玉芹,徐珍.维生素C对非洲爪蟾卵母细胞ATP-激活电流的增强作用[J].医学研究杂志,2010,39(7):49-51 |
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| 基金项目:湖北省教育厅重点科研项目（D20081108） |
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| 中文摘要:目的研究维生素C对非洲爪蟾卵母细胞内源性ATP受体激活电流（IATP）的调制作用。方法采用双电极电压钳技术，记录细胞外液中加入不同浓度维生素C时，对外加ATP所引起的卵母细胞膜IATP的影响。结果10-4 ～3×10-2mol/L浓度的维生素C 对10-4 mol/L IATP呈增强效应，其中以3×10-3 mol/L维生素C对IATP（尤其是D1部分）的增幅最大，可使IATP(D1)幅值增加（72.1±6.5）％, 显著高于对照值（P<0.01）；并使IATP量-效曲线左上移，但并不改变反应的最大幅值。结论本实验表明维生素C对ATP-激活电流的调制作用呈明显的增强效应，这种效应可能是通过对P2X受体-通道复合体活性调节的结果。而维生素C对不同的ATP受体亚型分别产生何种影响，还有待于进一步的研究。 |
| 中文关键词:维生素C ATP-激活电流 爪蟾卵母细胞 双电极电压钳技术 |
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| Augmentation by VitaminC on ATP-activated Currents in Xenopus Oocytes |
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| Abstract:ObjectiveTo study the modulatory effects of Vitamin C on ATP-activated Currents (IATP) of Xenopus oocytes. MethodsThe two electrode-voltage clamp technique was used to record IATP in healthy Xenopus oocytes by applying vitamin C in extracellular fluid. ResultsWe found that 10-4 mol/L IATP (especially the component D1) could be enhanced by Vitamin C from 10-4 mol/L to 3×10-2mol/L. The amplitude of IATP could be obviously increased by (72.1±6.5)％ when modulated by 3×10-3mol/L vitamin C, which showed a remarkable difference compared with the control value (P<0.01). Vitamin C could cause the concentration-response relationship curve to move towards up-left direction, while did not change the maximal amplitude. ConclusionThe modulation of Vitamin C on IATP in xenopas oocytes is enhancement effect. It is possible that such modulation results from activation of ATP receptor-channel complexes. If vitamin C has different effect on various ATP receptor subtypes,the corresponding questions can be answered in further studies. |
| keywords:Vitamin C ATP-activated current Xenopus oocytes Two-electrode voltage clamp |
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