| 虎杖苷吸入剂对哮喘大鼠肺组织TNF-α和Eotaxin基因表达的影响 |
| 投稿时间：2010-02-24 修订日期：2010-04-09 点此下载全文 |
| 引用本文：江庆澜,雷秀霞,梁成结,连家燕,巫小莉,刘桂长,梁颜笑.虎杖苷吸入剂对哮喘大鼠肺组织TNF-α和Eotaxin基因表达的影响[J].医学研究杂志,2010,39(7):62-65 |
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| 基金项目:广州市医药卫生科技项目（2008-YB-027）；广州市中医药、中西医结合科技项目（2009-A-05） |
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| 中文摘要:目的分析虎杖苷吸入剂对支气管哮喘大鼠模型TNF-α和Eotaxin基因表达调控的作用机制和干预效果。方法以10%的卵蛋白（OVA）和3%的氢氧化铝混合液腹腔注射实验大鼠2次致敏， 然后以2%的OVA诱喘，并进行虎杖苷和氨茶碱干预及生理盐水对照实验两周。通过RT-QPCR方法检测各组实验大鼠肺组织TNF-α mRNA和Eotaxin mRNA的相对水平，以t检验统计分析各项实验数据。结果虎杖苷干预组大鼠的哮喘症状得到明显缓解，其肺组织TNF-α和Eotaxin mRNA的相对水平（0.0005±0.00039, 0.0006±0.00035）比哮喘对照组（0.0165±0.01268, 0.0280±0.02488）和正常对照组(0.0017±000101, 0.0055±0.00209)均有所下降,但差异不显著（P>0.05, P>0.05; P>0.05, P>0.05）。结论虎杖苷吸入剂可降低哮喘大鼠肺组织的TNF-α和Eotaxin基因表达水平，有助于改善哮喘症状。 |
| 中文关键词:支气管哮喘 虎杖苷 肿瘤坏死因子α 嗜酸性粒细胞趋化因 子基因表达 |
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| Effects of Polydatin Inhalation on the TNF-α and Eotaxin Gene Expression in the Lung Tissue of Asthmatic Rats |
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| Abstract:ObjectiveTo analyze the effects and regulating mechanisms of TNF-α and Eotaxin gene expression of bronchial asthma rats intervened with polydatin inhalation. MethodsThe rats were injected with 1ml of the mix solution of 10% OVA and 3% aluminum hydroxide twice. And then, they were induced bronchial asthma with 2% OVA, and intervened with the polydatin solution and aminophyllin injection or controlled with saline for 2 weeks. The relative level of TNF-α and Eotaxin mRNA was detected by the methods of reverse transcription (RT) and real-time quantitative polymerase chain reaction (Real-time qPCR). The data differences between the groups were analyzed by t-test. ResultsThe asthmatic symptom of rats in polydatin intervenient group was obviously improved. Their relative level of TNF-α and eotaxin mRNA in lung tissue (0.0005±0.00039, 0.0006±0.00035) was lower than those of asthmatic group (0.0165±0.01268, 0.0280±0.02488) and control group (0.0017±0.00101, 0.0055±0.00209), but these differences between the groups were not significant （P>0.05, P>0.05; P>0.05, P>0.05）. ConclusionThe polydatin inhalation can decrease the TNF-α and eotaxin gene expression in the lung tissue of asthmatic rats to improve their asthmtic symptom. |
| keywords:Bronchial asthma Polydatin Tumor necrosis factor α Eotaxin Gene expression |
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