| 两种培养液对Beagle犬牙龈成纤维细胞生物学活性的影响 |
| 投稿时间：2010-03-28 修订日期：2010-05-26 点此下载全文 |
| 引用本文：钟泉,闫福华,李艳芬.两种培养液对Beagle犬牙龈成纤维细胞生物学活性的影响[J].医学研究杂志,2010,39(8):42-45 |
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| 基金项目:国家自然科学基金（30471892）、福建医科大学重点学科建设学术发展基金［闽医大口腔（2008）39号］ |
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| 中文摘要:目的探讨两种常见培养液：DMEM和RPMI1640对Beagle犬牙龈成纤维细胞生物学活性的影响。方法使用半消化+改良组织块法原代培养Beagle犬牙龈成纤维细胞，了解两种培养液下Beagle犬牙龈成纤维细胞游出成功率的差异；使用MTT、流式细胞仪检测两种培养液下细胞增生的情况；使用免疫细胞化学的方法检测两种培养液下Beagle犬牙龈成纤维细胞体外表达Ⅰ型胶原的差异。结果DMEM和RPMI1640对Beagle犬牙龈成纤维细胞的游出成功率、增生以及Ⅰ型胶原的表达均无显著性差异。结论DMEM和RPMI1640两种培养液均适合Beagle犬牙龈成纤维细胞的培养。 |
| 中文关键词:DMEM RPMI 1640 牙龈成纤维细胞 生物学活性 |
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| Effects of Tow Media on Bioactivities of Gingival Fibroblasts of Beagle Dog |
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| Abstract:ObjectiveTo observe the effects of tow media, DMED and RPMI 1640 on bioactivities of gingival fibroblasts of Beagle dog. MethodsThe gingival fibroblasts of Beagle dog were isolated, cultured and purified by half-digestion and improved tissue block culture.The difference of successful rate of gingival fibroblasts′ emigration under the two media was detected by repeated experiments. The differences of proliferation and expression of collagen I were investigated by MTT (flow cytometry) and immunocytochemistry respectively. ResultsThere was no significant difference between DMEM and RPMI1640 in successful rate of emigration, proliferation and expression of collagen Ⅰ. ConclusionThe two media, DMEM and RPMI1640 were both fit for the culture of gingival fibroblasts. |
| keywords:DMEM RPMI 1640 Gingival fibroblasts Bioactivities |
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