| 炎症和氧化应激标志物在犬心房颤动模型中的变化及意义 |
| 投稿时间：2010-03-09 点此下载全文 |
| 引用本文：官媛,郑强荪,单兆亮,李运明,郭红阳,张晔.炎症和氧化应激标志物在犬心房颤动模型中的变化及意义[J].医学研究杂志,2010,39(8):62-65 |
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| 中文摘要:目的分析犬心房颤动（房颤）模型炎症和氧化应激标志物的动态变化，探讨房颤的发病机制。方法24只杂种犬随机分为快速心房刺激组 (RAP) 和对照组，每组12只。RAP组给予右心房快速刺激(800次/分)，诱发并维持房颤2h。对照组不接受刺激。分别在基础状态、刺激后1h、2h测定右心房ERP和房颤诱发指数（AFII）、血清炎症 (TNF-α、IL-6) 和氧化应激标志物 (XO、GSH-Px)的水平。结果与对照组相比，①房颤后RAP组右房ERP显著缩短(P<0.01)；②在刺激1h时，RAP组XO水平即开始显著增高 (P<0.01)，GSH-Px含量显著减少 (P<0.05)，2 h时两者分别达到最大和最小值。TNF-α、IL-6在刺激2h时方出现显著增高(P<0.05)；③刺激1h和2h的AFII均与XO呈正相关，而与GSH-Px呈显著负相关。刺激1h时，AFII与炎症指标无显著相关，刺激2h时，AFII与IL-6、TNF-α呈正相关。刺激1h和2h的AERP200ms与AFII呈显著负相关。结论炎症与氧化应激参与了房颤发生和电重构过程，血清氧化应激标志物（XO和GSH-Px）可作为房颤早期预测指标。 |
| 中文关键词:心房颤动 犬 电重构 炎症氧化应激 |
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| The Changes and Significance of Inflammatory and Oxidative Stress Markers in Dog Model of Atrial Fibrillation |
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| Abstract:ObjectiveTo investigate the mechanisms of pathogenesis of AF by analyzing dynamic changes of inflammatory and oxidative stress markers in dog model of AF induced by rapid atrial pacing. MethodsTwenty-four dogs were randomly divided into rapid artrial pacing (RAP) group and normal control group. In the RAP group the right atrium was paced at 800 bpm to induce and maintain AF for 2 hours.The atrial electrophysiological indexes (AERP, AF induced rate), inflammatory markers (TNF-α, IL-6) and oxidative stress markers ( XO, GSH-Px ) in serum were measured at different time point(baseline, 1 h and 2 h after pacing). Results①The AERP shortened dramatically and AF induced rate increased significantly after 1 hour pacing in RAP group (P<0.01) ;②After 1 hour pacing, in RAP group, the level of oxidative index (XO) increased (P<0.01), while the level of anti-oxidation index ( GSH- Px ) dropped (P<0.05),and the two indexes reached maximum and minimum respectively at 2h. The levels of TNF-α and IL-6 had no significant changes at 1h, but increased markedly after 2 hours pacing in RAP group.③At 1h and 2h, the level of both XO and GSH significantly correlated with AF induced rate(XO: r1h=0.74, P<0.01；r2h=0.68, P<0.05; GSH: r=-0.78, P<0.01；r2h=0.83, P<0.01)). But a significant positive correlation could be found only after 2h pacing between inflammatory markers (TNF-α, IL-6) and AF induced rate (r=067，0．71，P<0．05) . At 1h and 2 h, AERP200ms was correlated with AF induced rate significantly(r1h=-0.69，P<0.05; r2h =-0.81，P<0.01). ConclusionOxidative stress and inflammatory reaction are closely associated with AF and electrical remodeling. Oxidative stress markers could predict the genesis of AF. |
| keywords:Atrial fibrillation Dog Electrical fibrillation Inflammation Oxidative stress |
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