| 胰岛素抑制自体移植静脉内皮细胞凋亡 |
| 投稿时间：2010-04-14 修订日期：2010-06-25 点此下载全文 |
| 引用本文：边杰芳,周宁,王廷,王海昌,高峰.胰岛素抑制自体移植静脉内皮细胞凋亡[J].医学研究杂志,2010,39(9):32-35 |
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| 基金项目:国家自然科学基金资助项目（30571827） |
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| 中文摘要:目的探讨胰岛素对移植静脉进行处理是否保护缺血、再灌注的自体移植静脉血管内皮细胞。方法32只杂种犬施行自体颈静脉-颈动脉移植术，术中将取出的移植静脉段分别浸入不同分组的处理液（每组8只）：肝素生理盐水（vehicle）组（saline：0.9% NaCl；肝素12500U/L）；GIK组（葡萄糖：250g/L；胰岛素：60U/L；KCl：80mmol/L；肝素12500U/L）；GK组（葡萄糖：250g/L； KCl：80mmol/L；肝素12500U/L）；GIK加渥曼青霉素（GIK+Wortmannin）组［处理液同GIK组，移植静脉再灌注前5min和后1~4h静脉注射渥曼青霉素2μg/（kg·min)］。术后4h获取移植静脉段进行凋亡蛋白caspase-3免疫组织化学测定和用末端脱氧核苷酸转移酶介导的dUTP原位平端标记法（TUNEL）定性和定量检测内皮细胞凋亡。结果与肝素生理盐水组相比，GK组增加移植静脉段内皮细胞和血管平滑肌细胞的凋亡(P<0.001)，而GIK组则显著减少凋亡(P<0.001)，渥曼青霉素则能有效抵抗胰岛素的抗凋亡作用。结论胰岛素对移植静脉段内皮细胞有保护作用，可能对移植静脉再狭窄有一定预防作用。 |
| 中文关键词:移植静脉 再狭窄 胰岛素 凋亡 |
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| The Antiapoptotic Effect of Insulin in Endothelial Cells of Autovenous Grafts |
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| Abstract:ObjectiveThis study was aimed at examining whether utilization of insulin exerted protective effect on the ischemic/reperfused canine autologous vein graft.MethodsThirty-two mongrel dogs were subjected to jugular-carotid interposition bypass grafting, and underwent infusions of vehicle (saline, 0.9% NaCl；heparin, 12500U/L), GIK (glucose, 250g/L; insulin, 60U/L; potassium, 80mmol/L; heparin, 12500U/L), GK or GIK plus wortmannin ［i.v, 2.0μg/(kg·min) for 1h immediately after reperfusion］ 5min before and 4h after reperfusion, respectively (n=8 in each group). All dogs were euthanized at 4h postoperationally to harvest vein grafts. Apoptosis of endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) in vein grafts were measured by active caspase-3 immunohistochemistry and TUNEL staining. ResultsCompared with vehicle, treatment with GK aggravated the apoptosis of ECs and VSMCs (P<0.001). However, treatment with GIK significantly decreased apoptosis of both ECs and VSMCs (P<0.001). Wortmannin largely abolished the GIK-elicited effects. ConclusionInsulin plays a protective role on autologous vein grafts, and likely prevents neointimal hyperplasia in autologous vein grafts. |
| keywords:Autovenous grafts Restenosis Insulin Apoptosis |
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