| GPC3启动子报告基因载体构建与转录活性分析 |
| 投稿时间：2010-04-27 点此下载全文 |
| 引用本文：金荣华,陈曦,王美霞,张洪海,孙玉,陈德喜.GPC3启动子报告基因载体构建与转录活性分析[J].医学研究杂志,2010,39(9):47-50 |
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| 基金项目:首都医学发展科研基金（2007-2050） |
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| 中文摘要:目的构建磷脂酰肌醇蛋白聚糖3(GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。方法以人类基因组DNA为模板，PCR扩增GPC3启动子基因片段，克隆到pGL3-Basic荧光素酶报告基因载体上，通过转染HepG2细胞检测荧光素酶活性来验证GPC3启动子的转录活性。结果GPC3基本启动子和全长启动子分别成功构建到pGL3-Basic荧光素酶报告基因载体中，GPC3基本启动子具有很强的转录活性，而全长启动子的转录活性比基本启动子高4倍以上。结论成功构建了GPC3启动子荧光素酶报告基因载体，为研究GPC3转录调控提供了重要手段。 |
| 中文关键词:GPC3 启动子 报告基因 荧光素酶 |
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| Construction of GPC3 Promoter Reporter Gene Vector and Analysis of its Transcriptional Activity |
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| Abstract:ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity. MethodsThe human genomic DNA was extracted as template.GPC3 promoter gene fragment was amplified and cloned into PGL3-Basic luciferase reporter gene vector. After HepG2 cells being transfected，the transcriptional activity of GPC3 promoter was verified through the detection of luciferase activity. ResultsBasal GPC3 promoter and full-length GPC3 promoter were successfully constructed to PGL3-basic luciferase reporter gene vector.GPC3 basal promoter had a strong transcriptional activity, while that of the full-length promoter was 4 times higher. ConclusionGPC3 promoter luciferase reporter gene vector was successfully constructed. |
| keywords:Glypican-3 Promoter Reporter gene Luciferase |
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