| 胸痹通胶囊调节血脂代谢的分子机制研究 |
| 投稿时间：2010-04-08 点此下载全文 |
| 引用本文：孙丽娜,王森林,唐可欣,张俊海,王桂芝.胸痹通胶囊调节血脂代谢的分子机制研究[J].医学研究杂志,2010,39(9):66-68 |
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| 基金项目:山东省潍坊市科技局计划课题（2006-2-47） |
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| 中文摘要:目的探讨胸痹通胶囊(XBTC)对高脂血症大鼠血脂代谢的影响及该药降脂作用的分子机制。方法①选取健康成年Wistar大鼠60只，随机分为正常对照组、高脂对照组和XBTC组，每组20只。分别饲以基础饲料、高脂饲料和高脂饲料加XBTC，12周后取大鼠血清，观察各组大鼠各项血脂指标；②选取健康成年Wistar大鼠40只，随机分为正常对照组、高脂对照组、XBTC组、血脂康药物对照组，每组10只。正常对照组给予正常饲料喂养；高脂对照组饲以高脂饲料；XBTC组给予高脂饲料加XBTC12g/(kg·d)；血脂康对照组给予高脂饲料加血脂康0.216g/(kg·d)。连续30天后，RT-PCR法测定大鼠肝低密度脂蛋白受体信使核糖核酸(LDLRmRNA)水平。结果①与正常对照组比较，高脂对照组血清胆固醇、三酰甘油明显升高为183mmol/L和1.09mmol/L，高密度脂蛋白(HDL-C)则明显降低为2.06mmol/L(P<0.05)，经XBTC干预后血脂异常得以纠正，血清胆固醇、三酰甘油分别降低为1.42mmol/L和0.70mmol/L，与高脂对照组比较，均有统计学意义(P<0.05)；②与高脂对照组比较，XBTC组能明显提高高脂血症大鼠肝LDLR mRNA水平(P<0.01)；与血脂康组比较无统计学意义(P>0.05)。结论XBTC心肌保护作用的机制可能与降低血脂代谢有关，其机制与明显增加高脂血症大鼠LDLR的活性，增强肝LDLR基因表达，加快LDL-C的清除有关。 |
| 中文关键词:血脂 低密度脂蛋白受体 胸痹通胶囊 大鼠 |
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| Study on the Molecular Mechanism of Xiongbitong Capsule in Regulating the Blood Lipid Metabolism |
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| Abstract:ObjectiveTo investigate the effect of xiongbitong capsule on blood lipids metabolism in hyperlipemia rats and study the molecular mechanism of xiongbitong capsule in decreasing serum lipid. Method①60 Wistar rats were divided into three groups randomly:the control group with normal food,hyperlipemia group with rich lipids food and treatment group with rich lipid food plus xiongbitong capsule 12g/(kg·d).After 12 weeks, blood sera were taken to observe the effects of xiongbitong capsule.②40 wistar rats were divided into 4 groups randomly including rats of control group fed with normal diet, rats of high lipid group fed with high fat diet,and rats of xiongbitong capsule group fed with high fat diet and xiongbitong capsule 12g/(kg·d), and rats of xuezhikang group fed with high fat diet and xuezhikang 0.216g/(kg·d). After 30 days,the levels of liver LDLR mRNA were detected by RT-PCR. Results①Compared with the normal control group, there was a significant increase in serum concentration of total cholesterol(TC),triglyceride(TG)(1.83,109mmol/L) and decrease in HDL (2.06mmol/L,P<0.05).The serum concentration of TC and TG decreased to 1.42 and 0.70mmol/L.②Compared with the higt fat group,the xiongbitong group can significantly increase the gene expression of LDLR mRNA in liver (P<001).But there was no significant different between the xiongbitong group and xuezhikang group(P>0.05).ConclusionXiongbitong capsule might have myocardial protective effect by correcting blood lipid. The effect of regulating serum lipid xiongbitong capsule might associate with its increasing the gene expression of liver LDLR and increasing the excretion of serum LDL-C. |
| keywords:Blood lipid Lower density lipoprotein receptor Xiongbitong capsule Rat |
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