| 北京地区哮喘儿童ORMDL3基因表达水平、生活方式与室内空气质量的相关性研究 |
| 投稿时间：2010-04-30 点此下载全文 |
| 引用本文：金哲,蔡欣,王强,李红,徐春雨,王秦,方建龙,高洁,付文亮,徐东群,徐东刚.北京地区哮喘儿童ORMDL3基因表达水平、生活方式与室内空气质量的相关性研究[J].医学研究杂志,2010,39(10):22-25 |
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| 基金项目:国家科技支撑计划项目课题（2006BAII9B05-3） |
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| 中文摘要:目的研究北京地区儿童ORMDL3基因表达水平、生活方式、室内空气质量与哮喘发生的相关性。方法采用病例-对照方法进行实验设计。使用RT-PCR法检测儿童ORMDL3基因表达水平；通过《国际儿童哮喘及过敏研究（ISSAC）问卷》收集儿童生活方式信息；并根据《室内空气质量标准 (GB/T18883-2002) 》中规定的检验方法对室内空气中PM2.5，VOCs，甲醛浓度及空气温湿度进行监测。使用t检验、卡方检验分析病例组与对照组儿童ORMDL3基因表达水平，生活方式与室内空气质量差异；使用Logistic逐步回归来分析ORMDL3基因表达水平，生活方式与室内空气质量对与哮喘之间相关性。结果ORMDL3基因表达水平在病例组与对照组中有显著差异（P<0.05）。在早发型哮喘患儿中，ORMDL3基因表达水平、哮喘家族史与哮喘显著相关(P<0.05)。而在迟发型哮喘患儿中，ORMDL3基因表达水平、采暖方式、室内空气湿度与哮喘显著相关（P<0.05）。结论ORMDL3基因表达水平与儿童哮喘的发生密切相关，早发型哮喘患儿与遗传因素有显著关联，而迟发型哮喘患儿中遗传因素与环境因素均与哮喘相关。 |
| 中文关键词:室内空气质量 生活方式 儿童哮喘 ORMDL3基因 病例对照队列 |
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| Study of Association between ORMDL3 Expression Level, Life-style Characters, Indoor Air Qualities and Childhood Asthma in Beijing |
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| Abstract:ObjectiveTo Study whether ORMDL3 expression level, life-style and indoor air quality were associated with childhood asthma in Beijing, China. MethodsWe used case-control design to perform our study. Life-style information was collected through ISAAC (International Study of Asthma and Allergies in Childhood) questionnaire results. RT-PCR was performed to test ORMDL3 expression levels. PM2.5, VOC, formaldehyde and humidity in bedrooms and living rooms were monitored by Indoor Air Quality Standard (GB/T18883-2002). T-test, chi-square and logistic regression with stepwise selection (SLE=0.2, SLS=0.2) analysis were used to test the association between ORMDL3 expression level, life-style, indoor air quality and childhood asthma. ResultsExpression level of ORMDL3 was associated with childhood asthma (P<0.05). ORMDL3 expression level and parent asthma history were associated with early-onset childhood asthma (P<0.05), while ORMDL3 expression level, the way of heating and humidity were associated with late-onset childhood asthma. ConclusionORMDL3 expression level was associated with childhood asthma in Beijing, China. Genetic factors were associated with early-onset childhood asthma, and both genetic factors and environmental factors were associated with late-onset asthma. |
| keywords:Indoor air quality Life-style characters Childhood asthma ORMDL3 Case-control cohort |
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