| Cdc2/cyclin B1 对中心体蛋白Nlp的调控在细胞生长中的作用 |
| 投稿时间：2010-09-25 修订日期：2010-10-20 点此下载全文 |
| 引用本文：赵雪莲,宋咏梅,金顺钱,詹启敏.Cdc2/cyclin B1 对中心体蛋白Nlp的调控在细胞生长中的作用[J].医学研究杂志,2010,39(12):25-29 |
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| 基金项目:国家重大基础研究计划“973”项目（2009CB521801）；国家自然科学基金重点项目和创新团体项目（30730046,30721001） |
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| 中文摘要:目的研究中心体蛋白Nlp 受Cdc2/cyclinB1磷酸化调控对细胞生长的影响。 方法Northern blot检测不同组织中Nlp的表达；EGFP-Nlp转染HeLa细胞，采用Double-Thymidine方法细胞同步化后释放，细胞免疫荧光方法观察Nlp在整个细胞周期中的亚细胞定位；构建Nlp磷酸化位点突变细胞系，采用人工计数和MTT法检测磷酸化位点突变对细胞生长的影响。结果Nlp在不同组织中存在表达差异，Nlp在不同细胞周期呈现不同的亚细胞定位，磷酸化位点Ser185和Ser589的突变促进细胞的体外生长。结论Cdc2/cyclin B1磷酸化位点突变后，Nlp获得了更强的促进细胞生长的能力。 |
| 中文关键词:Nlp Cdc2/cyclin B1 磷酸化 细胞生长 |
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| The Effect of Cdc2/cyclinB1 on Regulating Centrosome Protein Nlp in Cell Growth |
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| Abstract:ObjectiveTo study the effect of Cdc2/cyclinB1 on regulating centrosome protein Nlp in cell growth. MethodsNlp expression in different tissues was detected by Northern blot. Transfection EGFP-Nlp was transfected into HeLa cells,and then synchronized by Double-Thymidine.Nlp subcellular localization throughout the cell cycle was investigated by immunofluorescence. Nlp phosphorylation sites mutant cell lines were built. Phosphorylation site mutation on cell growth was detected by manually counting and MTT assay. ResultsNlp expression was distinct in different tissue. Nlp showed different subcellular localization throughout the cell cycle. Phosphorylation sites Ser185 and Ser589 mutation promoted cell growth in vitro. ConclusionNlp obtains stronger ability to promote cell growth after Cdc2/cyclin B1 phosphorylation sites mutant. |
| keywords:Nlp Cdc2/cyclin B1 Phosphorylation Cell growthBRCA1 |
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