| 多烯磷脂酰胆碱改善脂肪肝大鼠肝脏脂质沉积的作用机制研究 |
| 投稿时间：2010-06-02 修订日期：2010-11-03 点此下载全文 |
| 引用本文：李红山,陈少东.多烯磷脂酰胆碱改善脂肪肝大鼠肝脏脂质沉积的作用机制研究[J].医学研究杂志,2010,39(12):73-77 |
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| 中文摘要:目的探讨多烯磷脂酰胆碱对高脂饮食脂肪肝大鼠肝脏脂质沉积的抑制效应及其机制。方法采用单纯高脂饮食诱导的大鼠脂肪肝模型。在造模6周后, 随机分为模型组和多烯磷脂酰胆碱组，分别灌胃给予饮用水和相应药物，疗程4周，实验结束时取材观察：①肝组织病理变化（HE染色）；②血清脂联素（adiponection,ADP），肝组织三酰甘油（triglyceride, TG）、游离脂肪酸(free fatty acid, FFA)、肝组织脂联素受体（adiponection receptor2, AdipoR2）、腺苷酸活化的蛋白激酶（AMP-Activated K inase, AMPK）、脂肪酸合成酶（fatty acid synthase, FAS）、乙酰辅酶A羧化酶（acety1 CoA carboxylase, ACCase）、丙二酰辅酶A（Malony1 CoA）含量的变化及其各指标间相关性分析。结果模型组肝组织出现程度不同的肝细胞脂肪变性及空泡样变。模型组肝组织TG、FFA、FAS、ACCase、Malony1-CoA含量较正常组显著升高（P<0.01），血清ADP与肝组织AdipoR2、AMPK显著降低（P<0.01）。多烯磷脂酰胆碱组的肝脏脂肪变性显著减轻，肝组织TG、FFA、FAS、ACCase、Malony1-CoA含量显著降低（P<001），血清ADP与肝组织AdipoR2、AMPK含量明显升高（P<0.01）。结论多烯磷脂酰胆碱对脂肪肝大鼠肝脏脂质沉积有高强度的抑制效应，其机制与改善脂肪酸代谢有关。 |
| 中文关键词:实验性脂肪肝 三酰甘油 游离脂肪酸 脂联素 多烯磷脂酰胆碱 |
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| Mechanisms of Polyene Phosphatidylcholine in Inhibiting Liver Iipid Depositing in Rats with Fatty Liver |
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| Abstract:ObjectiveTo discuss the efficiency and mechanism of polyene phosphatidylcholine in inhibiting liver lipid depositing in rats with fatty liver induced by high-fat diet. MethodsSD male rats were used to establish NAFL model by high-fat diet for 10 weeks. After six weeks, the rats were respectively divided into model group and polyene phosphatidylcholine group by random, and were given corresponding medicine or drinking water via gastrogavage. After the rats were sacrificed, blood and livers were collected for research. The followings were tested:(1) liver pathology; (2) the adiponection content in serum,and triglyceride, free fatty acid, adiponection receptor 2, AMP-activated Kinase, acety1 CoA carboxylase, fatty acid synthase and malony1-CoA content in liver tissue.The correlation analysis among all the data above was studied. Results(1) There was serious hepatic steatosis in model group. The triglyceride, free fatty acid, acety1 CoA carboxylase, fatty acid synthase and malony1-CoA content in liver tissue of model group were elevated significantly, while the adiponection content in serum and AMP-activated kinase, adiponection receptor 2 content in liver tissue were reduced markedly. Polyene phosphatidylcholine could reduce pathological changes significantly. The triglyceride, free fatty acid, acety1 CoA carboxylase, fatty acid synthase, malony1-CoA content in liver tissue of polyene phosphatidylcholine group were reduced significantly, and the adiponection content in serum and AMP-activated kinase, adiponection receptor 2 content in liver tissue of polyene phosphatidylcholine group were raised markedly. (2) Triglyceride and free fatty acid content in liver tissue were negatively correlated with adiponectin content in serum, AMP-activated kinase, adiponectin receptor 2 content in liver tissue, while were positively correlated with fatty acid synthase, acety1 CoA carboxylase, malony1-CoA content in liver tissue. ConclusionPolyene phosphatidylcholine has strong inhibitory effect on liver lipid depositing in rats with fatty liver.The mechanism may be based on improving the fatty acid metabolism. |
| keywords:Experimental fatty liver Triglyceride Free fatty acid Adiponection Polyene phosphatidylcholine |
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