| 太白楤木药材质量标准研究 |
| 投稿时间：2010-07-06 修订日期：2010-09-20 点此下载全文 |
| 引用本文：李瑾,李扬,郭东艳,崔春利.太白楤木药材质量标准研究[J].医学研究杂志,2010,39(12):82-84 |
| DOI：
|
| 摘要点击次数: 1211 |
| 全文下载次数: 897 |
|
| 基金项目:陕西省教育厅资助项目(09JS015);陕西省中医药管理局资助项目(ZY31);陕西省重点学科专项建设资金资助 |
|
| 中文摘要:目的建立太白楤木药材中齐墩果酸的薄层色谱鉴别与含量测定方法。方法采用TLC法对太白楤木进行薄层鉴别；采用HPLC对太白楤木中齐墩果酸进行含量测定。结果该鉴别方法专属性强、重复性好，可作为该药材的薄层鉴别方法；含量测定的色谱条件为：色谱柱Kromosil ODS C18（5μm，4.6mm×250mm），甲醇-水（90∶10）为流动相，检测波长210nm，流速1.0ml/min，柱温为室温。齐墩果酸在44.8～224.0μg的范围内线性关系良好（r=0.9998），平均回收率为97.69%，RSD为123%。结论所建方法简便快捷，结果可靠，能够为控制太白楤木药材的质量提供参考。 |
| 中文关键词:太白楤木 薄层色谱鉴别 齐墩果酸 HPLC |
| |
| Study on Quality Control of Root Bark of Aralia Taibaiensis |
|
|
| Abstract:ObjectiveTo establish the TLC identification and quantitative determination methods of Aralia taibaiensis. MethodsTLC and HPLC were adopted to identify and determine Aralia taibaiensis. ResultsThis method had strong specificity and repeatability,and thus could be used as a TLC identification of this medicine. The chromatographic conditions were the follouing:Kromosil ODSC18（5μm，4.6mm×250mm）column as analytic column，methanol- water solution(90∶10)as mobile phase，detection wavelength at 210nm,flow rate at 1.0ml/min,column temperature at indoor temperature. The liner range of quercetin was 44.8～224.0μg(r=0.9998).The average recovery was 97.69%,with RSD=1.23%. ConclusionThe method and results are reliable and convenient for control the quality of Aralia taibaiensis for reference. |
| keywords:Aralia taibaiensis TLC Oleanolic acid HPLC |
| 查看全文 查看/发表评论 下载PDF阅读器 |
