| 巨细胞病毒肝炎患儿IL-12p40 3′非翻译区基因多态性研究 |
| 投稿时间：2010-07-24 点此下载全文 |
| 引用本文：叶松道,郑晓群,杨锦红,李伟,石海矾.巨细胞病毒肝炎患儿IL-12p40 3′非翻译区基因多态性研究[J].医学研究杂志,2010,39(12):92-96 |
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| 中文摘要:目的研究巨细胞病毒（CMV）肝炎患儿IL-12p40 3′非翻译区 rs3212227位点的基因型分布，探讨该位点的单核苷酸多态性与婴儿CMV肝炎发生的关系。 方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术和基因测序方法测定105例健康儿童及122例CMV肝炎患儿IL-12p40 3′非翻译区 rs3212227位点基因型，采用双抗体夹心ELISA法、速率法和实时荧光定量聚合酶链反应（FQ-PCR）分别测定CMV肝炎患儿血清IL-12p40水平、ALT活性及尿HCMV DNA载量。 结果CMV肝炎组IL-12p40 rs3212227位点AA、AC、CC基因型分布频率分别为34.4%、41.8%、23.8%，正常对照组分别为476%、40.0%、12.4%，两组间CC基因型和非CC基因型之间频率分布差异有统计学意义（χ2=4.855，P<0.05，OR CC=2.207，95%CI：1.080~4.510）。CMV肝炎组IL-12p40 rs3212227位点等位基因A、C分布频率分别55.3%、44.7%，对照组分别为676%、32.4%，两组等位基因频率比较差异有统计学意义（χ2=7.168，P<0.01；OR A =0.593，95%CI：0.404~0.871；ORC=1686，95%CI：1.149~2.475）。 rs3212227位点不同基因型的CMV肝炎患儿之间血清IL-12p40水平、尿HCMV DNA载量（拷贝/毫升,lg）差异均有统计学意义（H=7.385、F=9.325，P均<0.05），CC基因型患儿血清IL-12p40水平低于AA基因型，尿液HCMV DNA载量高于AA基因型，不同基因型患儿之间血清ALT活性差异无统计学意义（P>0.05）。结论IL-12p40 3′非翻译区rs3212227位点单核苷酸多态性与婴儿CMV肝炎的发生具有相关性，其中C等位基因可能是其遗传易感基因，携带C等位基因的个体可能更利于CMV病毒的复制。 |
| 中文关键词:巨细胞病毒肝炎 基因型 多态性 单核苷酸 白细胞介素12p40 |
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| Study on the Gene Polymorphism of IL-12p40 3′ Untranslated Region in Infant with Cytomegalovirus Hepatitis |
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| Abstract:ObjectiveTo investigate the single nucleotide polymorphism(SNP) of IL-12p40 3′ untranslated region (3′ UTR) rs3212227 site in cytomegalovirus hepatitis infants，and find the association between IL-12p40 SNP and cytomegalovirus hepatitis in China. MethodsWe genotyped IL-12p40 rs3212227 SNP of 122 cytomegalovirus hepatitis infants and 105 healthy controls by PCR-RFLP. We also detected IL-12p40 level,ALT activity in serum and HCMV DNA in urinary of cytomegalovirus hepatitis infants. ResultsThe frequency of AA,AC and CC genotype of IL-12p40 rs3212227 site were 34.4%，41.8% and 23.8% in CMV hepatitis infants， and the frequency were 47.6%,40.0% and 12.4% in control group.And there was significant difference in CC genotype and non-CC genotype frequency between the two grouops（χ2 =4.855，P<0.05，OR CC =2.207，95%CI：1.080~4.510）. The frequency of A,C allele of IL-12p40 rs3212227 site were 55.3%,44.7% in CMV hepatitis infants，and were 67.6%,32.4% in control group. A significant difference was found in allele frequency distribution of IL-12p40 rs3212227 between the two groups（χ2 =7.168，P<0.01；OR A=0593， 95%CI：0.404~0.871； OR C =1.686，95%CI：1.149~2.475）.There were signincant differences in HCMV DNA in urinary,IL-12p40 level in serum between AA,AC and CC genotype of CMV hepatitis infants（P<0.05），but there was no significant difference in ALT activity between the three genotype of CMV hepatitis infants. ConclusionThere was a relationship between the SNP of IL-12p40 3′ UTR rs3212227 and the susceptibility to CMV hepatitis in infants.The infants with C allele and CC genotype are susceptible to develop CMV infection. |
| keywords:Cytomegalovirus hepatitis Genotype Single nucleotide polymorphism Interleukin-12p40 |
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