| B7-H4在人类肝癌细胞株中的表达及药物作用后其在BEL-7404中的表达变化 |
| 投稿时间：2010-09-16 修订日期：2010-11-15 点此下载全文 |
| 引用本文：杜振东,于嘉屏,徐晓萍,林炜炜,徐磊,彭凤.B7-H4在人类肝癌细胞株中的表达及药物作用后其在BEL-7404中的表达变化[J].医学研究杂志,2011,40(2):30-33 |
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| 中文摘要:目的研究B7家族的新成员B7-H4 mRNA在肝癌细胞株中的表达，以及BEL-7404在抗肿瘤药物索拉非尼作用下mRNA的表达变化。方法采用反转录聚合酶连反应( RT-PCR) 方法检测10株肝细胞株中mRNA的表达；使用不同浓度索拉非尼杀伤BEL-7404后检测其B7-H4 mRNA的表达。 结果B7-H4 mRNA在部分肝癌细胞株中有表达；在使用索拉非尼后BEL-7404 B7-H4表达明显下降。结论B7-H4基因与肿瘤细胞生长活性密切相关。 |
| 中文关键词:肝细胞癌 B7-H4 肿瘤免疫 索拉非尼 |
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| The Expression of B7-H4 mRNA in HCC Cell Lines and the Changes in BEL-7404 after Using Sorafenib |
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| Abstract:ObjectiveTo study the mRNA expression of B7-H4 in hepatocellular carcinoma(HCC) cell lines and the expression changes in BEL-7404 under anticancer drug sorafenib. MethodsThe mRNA expression of B7-H4 in ten liver cell lines was detected by reverse transcription polymerase chain reaction(RT-PCR).With different concentrations sorafenib, we observed the change of B7-H4 mRNA expression in BEL-7404. ResultsB7-H4 mRNA was expressed partly in these liver cancer cell lines. B7-H4 mRNA of BEL-7404 was significantly decreased after using sorafenib. ConclusionB7-H4 gene is closely related to tumor cell growth activity. |
| keywords:Hepatocellular carcinoma B7-H4 Tumor immunity Sorafenib |
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