| 银杏叶提取物对肝纤维化大鼠肝脏Smad2/3表达的影响 |
| 投稿时间：2010-07-20 点此下载全文 |
| 引用本文：姚嘉明,陈芝芸,严茂祥,高晓倩,孟爱红.银杏叶提取物对肝纤维化大鼠肝脏Smad2/3表达的影响[J].医学研究杂志,2011,40(2):37-39 |
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| 基金项目:浙江省中医药重点研究项目（2006Z006）；浙江省中医药科技计划（2009YA006） |
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| 中文摘要:目的观察银杏叶提取物（GbE）对肝组织Smad2/3表达的影响，探讨GbE抗肝纤维化的作用机制。方法采用CCl4诱导大鼠肝纤维化，同时分别以200、100、50mg/(kg·d)的GbE进行干预，并设复方鳖甲软肝片为对照组，6周后取肝组织，原位杂交及免疫组化技术分别检测肝组织Smad2/3mRNA及蛋白的表达。结果模型组大鼠肝组织Smad2/3mRNA及蛋白的表达正常组明显增高，应用不同剂量的GbE及复方鳖甲软肝片干预后肝组织Smad2/3mRNA及蛋白较模型组显著下降。结论GbE降低Smad2/3mRNA及蛋白表达可能是其抗肝纤维化作用的重要机制之一。 |
| 中文关键词:银杏叶提取物 肝纤维化 Smad2/3 |
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| Effects of Ginkgo Biloba Extract on Expression of Smad2/3 in Rats with Liver Fibrosis |
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| Abstract:ObjectiveTo evaluate the effects of Ginkgo biloba extrat(GbE) on expression of Smad2/3,thus to investigate the possible mechanism of GbE in rats with hepatic fibrosis. MethodsThe liver fibrosis model was induced by CCl4. The rats in administration groups were given with 200mg/kg,100mg/kg,50mg/kg of GbE by daily gavage.At the end of the sixth week, all the rats were sacrificed and livers were obtained. The expression of Smad2/3 was detected by immunohistochemistry. The expression of Smad2/3mRNA in liver was measured by hybridization in situ. ResultsThe expression of Smad2/3mRNA and Smad2/3 protein were significantly reduced after the administration of GbE and Fufang Biejia Ruangan Pian in model group. ConclusionGbE could reduce the transcription and expression of Smad2/3, and inhibit the occurrence and development of CCl4-induced liver fibrosis. |
| keywords:Ginkgo biloba extrat Liver fibrosis Smad2/3 |
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