| 筋脉通对糖尿病大鼠坐骨神经NADPH氧化酶p22-phox亚基表达的影响 |
| 投稿时间：2010-08-24 修订日期：2011-03-27 点此下载全文 |
| 引用本文：李伯武,梁晓春,王普艳,赵丽,黄文智.筋脉通对糖尿病大鼠坐骨神经NADPH氧化酶p22-phox亚基表达的影响[J].医学研究杂志,2011,40(5):43-46 |
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| 基金项目:北京市自然科学基金资助项目（7082077） |
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| 中文摘要:目的观察中药筋脉通对糖尿病大鼠坐骨神经NADPH 氧化酶p22-phox亚基表达的影响。方法将STZ诱导的糖尿病大鼠随机分为模型组，维生素C组，筋脉通小、中、大剂量组，并设立正常对照组，连续灌胃16周。检测各组治疗前及治疗后 4、8、12、16周的体重、血糖；测定第16周时大鼠机械痛阈值；采用免疫组化法测定大鼠坐骨神经NADPH 氧化酶p22-phox亚基的表达，并进行半定量分析。结果与正常组相比，各组糖尿病大鼠体重均显著下降(P<0.01)，血糖均明显升高(P<0.01)；与模型组比较，各治疗组大鼠体重及血糖在各时间点均无显著差异（P>0.05）。与正常组相比，模型组、VC组、筋脉通小、大剂量组机械痛阈值显著降低（P<0.01）；各治疗组机械痛阈值较模型组均明显升高（P<0.01）；与VC组比较，筋脉通中剂量组机械痛阈值显著升高（P<0.01）。与正常组相比，模型组及各治疗组p22亚基的IOD值明显升高（P<0.05，P<0.01）；各治疗组p22亚基的IOD值较模型组均显著降低（P<0.01）；与VC组比较，筋脉通中、大剂量组p22亚基IOD值显著降低（P<0.01，P<0.05）。结论筋脉通能显著降低大鼠坐骨神经NADPH 氧化酶p22-phox亚基的表达。 |
| 中文关键词:筋脉通 糖尿病周围神经病变 NADPH氧化酶 p22-phox亚基 |
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| Effect of Jin Mai Tong on p22-phox Subunit Expression of NADPH Oxidase in Sciatic Nerve of Diabetic Rats |
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| Abstract:ObjectiveTo observe the effect of JinMaiTong(JMT) on p22-phox subunit expression of NADPH Oxidase in sciatic nerve of DM rats. MethodsDiabetic rats induced by streptozotocin(STZ) were randomly divided into model group, vitamin C group, low-dose,moderate-dose and high-dose JMT group in addition to control group. All rats were given intragastric administration for 16 weeks. Body weight and blood glucose were detected before the treatment and at the 4th, 8th, 12th, 16th week after treatment. Pain threshold was measured at the 16th week.The expression of p22-phox subunit in sciatic nerves was quantified by SABC immunohistochemical method and then analyzed with semi-quantitative method. ResultsCompared with control group, the body weight level of STZ-DM rats decreased significantly (P<0.01) while the scale of blood glucose raised strikingly (P<0.01). In all treatment groups, there were no significant differences in body weight levels or blood glucose levels compared with model group at any time (P>0.05).Compared with control group, the pain threshold level of model group,VC group,low-dose and high-dose JMT group reduced distinctly (P<0.01), while the integrated option density(IOD) scale of p22-phox subunit expression of model group and treated groups increased obviously（P<0.05，P<0.01）.The threshold scale of all treated groups was higher than model group（P<0.01）, while the IOD level was lower than model group（P<0.01）. Compared with VC group, moderate-dose JMT group experienced a raise of the threshold level （P<0.01）,while moderate-dose and high-dose JMT group reduced in the IOD scale of p22-phox subunit expression（P<0.01，P<0.05）.ConclusionJMT is effective in reducing p22-phox subunit expression level of NADPH oxidase in sciatic nerve of DM rats. |
| keywords:Jin Mai Tong Diabetic peripheral neuropathy NADPH oxidase p22-phox subunit |
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