| 全反式维甲酸对睾丸卵黄囊瘤细胞VEGF表达的抑制作用 |
| 投稿时间：2010-11-20 点此下载全文 |
| 引用本文：张浩川,包小周,陈聪德,李仲荣,陈肖鸣.全反式维甲酸对睾丸卵黄囊瘤细胞VEGF表达的抑制作用[J].医学研究杂志,2011,40(6):53-56 |
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| 基金项目:浙江省自然科学基金资助项目(Y204463)；浙江省卫生厅卫生科技重点项目(2004ZD009) |
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| 中文摘要:目的研究全反式维甲酸（ATRA）对睾丸卵黄囊瘤细胞血管内皮生长因子（vascular endothelial growth factor，VEGF)表达的抑制作用。方法在体外细胞培养的基础上,采用MTT比色法测定不同浓度ATRA对睾丸卵黄囊瘤细胞增殖影响,半定量RT-PCR方法检测VEGF mRNA的表达水平，Western Blot法检测VEGF蛋白表达水平。结果ATRA能抑制肿瘤细胞增殖，RT-PCR结果显示实验组卵黄囊瘤细胞的VEGF mRNA表达水平下调，Western Blot显示实验组卵黄囊瘤细胞的VEGF蛋白表达水平降低，且两者下调作用均呈较明显的时间-剂量依赖性。结论全反式维甲酸可以抑制睾丸卵黄囊瘤细胞的增殖，抑制肿瘤增殖机制可能与下调VEGF的表达有关。 |
| 中文关键词:全反式维甲酸 睾丸卵黄囊瘤细胞 体外培养 VEGF |
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| VEGFEffects of All-trans Retinoic Acid on the Expression of VEGF in Testicular Yolk Sac Tumor Cell Line in vitro |
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| Abstract:ObjectiveTo investigate the inhibitory effects of all-trans retinoic acid on the expression of VEGF in testicular yolk sac tumor cell line in vitro. MethodsBy technique of cell culture in vitro,testicular yolk sac tumor cell line was exposed to ATRA of different concentration. MTT was used to examine the effects of ATRA on proliferation of testicular yolk sac tumor cell line.The VEGF mRNA expression levels were determined by semi-quantitative RT-PCR. The protein expressions of VEGF were analyzed by Western blot.ResultsAfter yolk sac tumor cells were treated with all-trans retinoic acid,the proliferation index decreased markedly.The mRNA and protein expressions of VEGF decreased dramatically in a time -dose dependent manner. ConclusionATRA could inhibit proliferation of yolk sac tumor cell line,and down regulation of VEGF could be the inhibitory mechanism of ATRA on yolk sac tumor cell line in vitro. |
| keywords:ATRA Testicular yolk sac tumor cell line Culture in vitro VEGF |
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