| 慢性乙型肝炎肝纤维化患者外周血CD4+CD25+CD127low/-T细胞与人肝星状细胞共培养的实验研究 |
| 投稿时间：2011-01-10 点此下载全文 |
| 引用本文：金晓芝,陈永平,程瑗,潘黎静,潘陈为,戴志娟,叶超.慢性乙型肝炎肝纤维化患者外周血CD4+CD25+CD127low/-T细胞与人肝星状细胞共培养的实验研究[J].医学研究杂志,2011,40(8):70-73 |
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| 基金项目:浙江省自然科学基金资助项目(Y207464) |
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| 中文摘要:目的探讨CD4+CD25+CD127low/- T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。 方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例；采用磁激活细胞分选法（MACS）提取13例患者和6例健康对照者外周血CD4+CD25+CD127low/- T细胞；分别将CD4+CD25+CD127low/- T细胞以不同比例与人肝星状细胞株(hepatic stellate cell，HSC)共培养5天，检测各组HSC的增殖及TGF-β1的分泌。 结果 CD4+CD25+CD127low/-T细胞与HSC之比，当健康对照组和S1~S2期组为1.5∶1，S3~S4期组为2∶1时，HSC的增殖速度和TGF-β1分泌量均在同组不同比例中达最高值。各组同比例之间，当比例为1.5∶1时，健康对照组HSC的增殖速度显著高于 S3~S4期组(P<0.05)；在比例为1∶1~2∶1范围内，S1~S2期组和S3~S4期组HSC的TGF-β1分泌量远高于同比例健康对照组(P<0.05)。 结论CD4+CD25+CD127low/-T细胞可能通过自身细胞数量和功能的双重调节，影响肝星状细胞的增殖和分泌，参与了慢乙肝肝纤维化的进展。 |
| 中文关键词:慢性乙型 病毒性肝炎 肝纤维化CD4+CD25+CD127low/- Treg MACS |
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| The Study of Co-culturing CD4+CD25+CD127low/- T Cells from Patients with HBV-related Liver Fibrosis and Hepatic Stellate Cells in vitro |
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| Abstract:ObjectiveTo investigate the roles of CD4+CD25+CD127low/-T cells in HBV-related liver fibrosis.MethodsFibrosis stage was confirmed by pathohistological examination of liver section.CD4+CD25+CD127low/- T cells were isolated respectively from peripheral blood of 6 healths,6 patients in S1-S2 stage and 7 patients in S3-S4 stage by MACS.We then cultured CD4+CD25+CD127low/- T cells and human hepatic stellate cell(HSC) with different ratios respectively,and after 5 days,appraised the proliferation of HSC by CCK8 and used Elisa method to test concentration of TGF-β1 secreted by HSC.ResultsWe discovered the strongest proliferation and the highest TGF-β1 secretion of HSC when the healths and patients in S1-S2 stage ratio of (the numbers of CD4+CD25+CD127low/- T cells/HSC) 1.5∶1,patients in S3-S4 stage of 2∶1.From health control group,we also found that HSC had a stronger proliferation compared with patients in S3-S4 stage when ratio was 1.5∶1(P<0.05).And the ratios ranged from 1∶1 to 2∶1.There was a lower TGF-β1 secretion of HSC in health control group compared with other groups(P<0.05).ConclusionChanges of numbers and functionalities of CD4+CD25+CD127low/- T cells might promote HSC proliferation and TGF-β1 secretion and contribute to progression of HBV- related liver fibrosis. |
| keywords:Chronic hepatitis B Liver fibrosis CD4+CD25+CD127low/- Treg MACS |
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