| 香烟烟雾暴露对大鼠肺组织CCR7和外周血MIP-3β表达的影响 |
| 投稿时间：2010-12-01 修订日期：2010-12-29 点此下载全文 |
| 引用本文：刘亮,许建英.香烟烟雾暴露对大鼠肺组织CCR7和外周血MIP-3β表达的影响[J].医学研究杂志,2011,40(8):118-121 |
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| 中文摘要:目的研究香烟烟雾暴露对大鼠肺组织CCR7和外周血MIP-3β表达的影响，探讨免疫细胞在吸烟所致气道慢性炎症中的作用。方法30只雄性Wistar大鼠随机分为不吸烟组、吸烟6周组、吸烟12周组；分别用免疫组化半定量法和酶联免疫吸附试验（ELISA）检测各组肺组织CCR7和外周血MIP-3β的含量。结果（1）与不吸烟组(0.173±0.037)比较，吸烟6周组(0.282±0.029) 和吸烟12周组(0.379±0.038)大鼠CCR7的表达明显增加，差异均有统计学意义(P均<0.01)；吸烟12周组CCR7表达与吸烟6周组比较有所增加，差异也有统计学意义(P＜0.01)。（2）与不吸烟组(77.94±18.59pg/ml)比较，吸烟6周组(162.51±15.66pg/ml)和吸烟12周组(228.34±26.18pg/ml)外周血中MIP-3β含量明显增加，差异均有统计学意义(P均＜0.01)；吸烟12周组MIP-3β与吸烟6周组比较有所增加，差异也有统计学意义(P＜0.01)。（3）肺组织CCR7与外周血MIP-3β的表达呈正相关（r=0.915）。结论吸烟可使大鼠CCR7和MIP-3β的表达水平增高，二者表达呈正相关，提示免疫细胞可能在吸烟所致气道慢性炎症中发挥作用。 |
| 中文关键词:香烟烟雾 气道慢性炎症 树突状细胞 CCR7 MIP-3β |
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| The Effect of Cigarette Smoke Exposure on Expression of CCR7 in Lung and MIP-3β in Peripheral Blood of Rats |
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| Abstract:ObjectiveTo explore the effect of cigarette smoke exposure on the expression of CCR7(Chemokine receptor7) in lung and MIP-3β（Macrophage inflammatory protein-3β）in peripheral blood of rats.MethodsThirty male (n=30)Wistar rats were randomly divided into three groups: non-smoking group,smoking 6 weeks group and smoking 12 weeks group.CCR7 was semiquantitatively analyzed in lung by immunohistochemistry.The concentration of MIP-3β in peripheral blood was determined by enzyme-linked immunosorbent assay (ELISA).Results（1）The expression of CCR7 in lung and MIP-3β in peripheral blood of rats in the smoking 6 weeks group （0.282±0.029，162.51±15.66pg/ml）and the smoking 12 weeks（0.379±0.038，228.34±26.18pg/ml） group were higher than those in non-smoking group（0.173±0.037，77.94±18.59pg/ml）(all P<0.01),with the smoking 12 weeks group higher than the smoking 6 weeks group (all P<0.01)（2）The levels of MIP-3β in peripheral blood positively related with CCR7.ConclusionCigarette smoke exposure can upregulate the expression of CCR7 protein in lung and the levels of MIP-3β in peripheral blood in rats,which indicates that immune cells may play an important role in chronic airway inflammation caused by smoking. |
| keywords:Cigarette smoke Chronic airway inflammation Dendritic cells CCR7 MIP-3β |
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