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CP-154,526和verapamil对促肾上腺皮质激素释放因子分泌水平的影响

投稿时间：2011-03-29 修订日期：2011-06-26 点此下载全文

引用本文：董静,祝益民,周文武,袁发,张川,董春艳,肖政辉,桂永浩,陈超.CP-154,526和verapamil对促肾上腺皮质激素释放因子分泌水平的影响[J].医学研究杂志,2011,40(9):66-68

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作者	单位
董静	湖南省儿童医院
祝益民	湖南省儿童医院
周文武	湖南省儿童医院
袁发	湖南省儿童医院
张川	湖南省儿童医院
董春艳	湖南省儿童医院
肖政辉	湖南省儿童医院
桂永浩	复旦大学儿科医院
陈超	复旦大学儿科医院

中文摘要:目的探讨促肾上腺皮质激素释放因子Ⅰ型受体（CRF-R1）拮抗剂CP-154,526（CP）和钙离子通道拮抗剂verapamil（VP）对幼年大鼠在缺氧缺血（HI）后并放置1天后血浆中促肾上腺皮质激素释放因子分泌水平的影响。方法80只幼年大鼠随机分成8组，即对照组、假手术组、CP对照组、VP对照组、模型组（HI组）、HI+CP组、HI+VP组和HI+CP+VP组。用放射免疫法测定各组幼年大鼠血浆中CRF水平。结果与对照组比较，HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低（P＜0.001、P＜0.001、P＜0.001）；与假手术组比较，HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低（P＜0001、P＜0.001、P＜0.001）；与CP对照组比较，HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低（P＜0.001、P＜0.001、P＜0.001）；与VP对照组比较，HI组、HI+CP组和HI+CP+VP血浆CRF水平都显著降低（P＜0.001、P＜0.001、P＜0001）；与HI组比较，HI+CP组和HI+VP组血浆CRF水平显著增加（P＜0.05、P＜0.001）；与HI+CP比较，HI+VP组血浆CRF水平显著增加（P＜0.001）；与HI+VP组比较，HI+CP+VP组血浆CRF水平显著降低（P＜0.001）。结论幼年大鼠在HI后并放置1天后血浆CRF水平都显著减少；可是，当幼年大鼠用CP或者VP预处理后，血浆CRF分泌水平在缺氧缺血下的减少能被逆转；当用CP和VP同时预处理后，血浆CRF分泌水平在缺氧缺血下的减少没有被改变。

中文关键词:缺氧缺血脑损伤促肾上腺皮质激素释放因子 CP-154,526 Verapamil 幼年SD大鼠

Effects of CP-154,526 and Verapamil on Corticotrophin Releasing Factor Secreting Levels

Abstract:ObjectiveTo explore the effects of CRF acceptor 1 (CRF-R1) antagonist CP-154,526 (CP) and Ca2+ pathway antagonist verapamil (VP) on plasma CRF secreting levels in the young rats after hypoxic-ischemia (HI) and after the rats were placed for one day.MethodsEighty young rats were randomly divided into eight groups,which were the control groups,sham operated groups,CP control groups,VP control groups,model groups (HI groups),HI+CP groups,HI+VP groups and HI+CP+VP groups.Plasma CRF levels of the young rats in all groups were measured by radioimmunoassay.ResultsCompared with the control groups,plasma CRF levels in the HI groups,HI+CP groups and HI+CP+VP groups all significantly decreased (P＜0.001,P＜0.001,P＜0.001).Compared with the sham operated groups,plasma CRF levels in the HI groups,HI+CP groups and HI+CP+VP groups all significantly decreased (P＜0.001,P＜0.001,P＜0.001).Compared with the CP control groups,plasma CRF levels in the HI groups,HI+CP groups and HI+CP+VP groups all significantly decreased (P＜0.001,P＜0.001,P＜0.001).Compared with the VP control groups,plasma CRF levels in the HI groups,HI+CP groups and HI+CP+VP groups all significantly decreased (P＜0.001,P＜0.001,P＜0.001).Compared with the HI groups,plasma CRF levels in the HI+CP groups and HI+VP groups all significantly increased (P＜0.05,P＜0.001).Compared with the HI+CP groups,plasma CRF levels in the HI+VP groups significantly increased (P＜0.001).Compared with the HI+VP groups,plasma CRF levels in the HI+CP+VP groups significantly decreased (P＜0.001).ConclusionPlasma CRF levels in the young rats after HI and after the young rats were placed for one day significantly decreased.But,after the young rats were pretreated with CP or VP,the decrease of plasma CRF secreting levels under HI can be inversed.After the young rats were simultaneously pretreated with CP and VP,the decrease of plasma CRF secreting levels under HI was not changed.

keywords:Hypoxic-ischemia brain injury Corticotrophin releasing factor CP-154,526 Verapamil Young SD rat

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