| siRNA沉默VEGF-C 基因对宫颈癌HeLa细胞的生物学特性影响 |
| 投稿时间：2011-03-23 修订日期：2011-10-08 点此下载全文 |
| 引用本文：陈星,王美芬,吴朝阳,郑灵芝,任虹平,郑曙民,熊冬生,杨纯正,糜若然.siRNA沉默VEGF-C 基因对宫颈癌HeLa细胞的生物学特性影响[J].医学研究杂志,2011,40(11):58-62 |
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| 基金项目:浙江省卫生厅科技基金资助项目（2008196B）；浙江省台州科技基金课题（08YK14） |
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| 中文摘要:目的通过RNA干扰技术抑制宫颈癌细胞VEGF-C表达，探讨干扰后VEGF-C、NF-κB、bcl-2基因的表达。方法根据人VEGF-C mRNA编码序列，设计RNA干扰的靶点，并用脂质体转染人宫颈癌HeLa细胞，通过RT-PCR法观察转染后肿瘤细胞VEGF-C、NF-κB、bcl-2基因的变化。结果转染siRNA 24h、48h可以使HeLa细胞VEGF-C mRNA含量降低，在24h降低80.63%±0.24% （P＜0.001）；在48h降低38.9%±0.85% （P＜0.01）；NF-κB mRNA含量也分别降低，在24h 降低37.55±2.76 % （ P＜0.05）；在48h降低30.5%±3.82%（P=0.056）；bcl-2mRNA含量同时分别降低，在24h降低7695%±191%，（P＜0.01）；在48h降低64.11%±2.96%，（P＜0.05））。结论脂质体介导的VEGF-C siRNA 转染HeLa细胞后，可以有效抑制VEGF-C的表达；可能通过下调转录因子NF-κB，抑制抗凋亡基因bcl-2的表达。 |
| 中文关键词:血管内皮生长因子-C bcl-2 NF-κB 宫颈癌 RNA干扰 |
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| Effect of Molecular Biology of Inhibition of VEGF-C in HeLa Cell Lines by siRNA in Vitro |
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| Abstract:ObjectiveTo analysis the expression of VEGF-C,NF-κB,bcl-2 gene by inhibiting VEGF-C expression of tumor cells with RNA interference （RNAi）technique.MethodsAccording to the encoding sequence mRNA of human VEGF-C,the target site for RNAi was designed，siRNA was transfected by liposome and the expression of VEGF-C,NF-κB,bcl-2 mRNA was further observed by RT-PCR. ResultsThe expression of VEGF-C of HeLa cell lines treated with siRNA for 24h,48h was significantly inhibited,with in 24h 80.63%±0.24% (P＜0.001) and in 48h 38.9%±0.85%(P＜0. 01）.The expression of NF-κB was also down-regulated in 24h,48h,with in 24h 37.55%±2.76%(P＜0.05) and in 48h 30.5%±3.82%(P=0.056).Thus the expression of bcl-2 was also inhibited in 24h,48h,with in 24h 76.95%±1.91% (P＜0.01) and in 48h 64.11%±2.96%(P＜0.05）.ConclusionAfter being treated with VEGF-C siRNA of the HeLa cells,the expression of VEGF-C is effectively inhibited,NF-κB may be down-regulated and anti-apoptosis bcl-2 gene is effectively inhibited. |
| keywords:Vascular endothelial growth factor-C bcl-2 RNA interference Cervical carcinoma |
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