| 雷帕霉素与环孢素A对人乳腺癌细胞的耐药逆转作用比较 |
| 投稿时间：2011-03-04 修订日期：2011-03-11 点此下载全文 |
| 引用本文：沈香娣,范兴丽,仇容,陈健,刘丹丹.雷帕霉素与环孢素A对人乳腺癌细胞的耐药逆转作用比较[J].医学研究杂志,2011,40(11):96-98 |
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| 基金项目:浙江省教育厅科研计划项目（Y200805993） |
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| 中文摘要:目的比较雷帕霉素与环孢素A对人乳腺癌细胞的耐药逆转作用。方法MTT法测定人乳腺癌耐药细胞株MCF-7/ADR的耐药倍数。乳腺癌敏感细胞株MCF-7/S和耐药细胞株MCF-7/ADR各自分为：对照组：加入不同浓度阿霉素；实验组：加入不同浓度的阿霉素+不同浓度的雷帕霉素或者不同浓度的阿霉素+不同浓度的环孢素A；空白组。MTT法观察细胞抑制率，并算得半数抑制浓度，得出耐药逆转倍数。用免疫荧光法测得加入阿霉素的MCF-7/ADR细胞株在不同浓度的雷帕霉素或者环孢素A作用下，细胞内的阿霉素浓度。结果（1）MCF-7/ADR细胞株的耐药倍数为12.13。（2）对于MCF-7/S细胞株，雷帕霉素和环孢素A的耐药逆转倍数均为1。（3）对于MCF-7/ADR细胞株，10μmol/L环孢素A使阿霉素的IC50显著降低（P<0.05），逆转倍数为1.6，并且随着浓度的增加，IC50逐渐降低，逆转倍数有逐渐增高的趋势；1μmol/L雷帕霉素使阿霉素的IC50显著降低（P<0.05），逆转倍数为1.6，并且随着浓度的增加，IC50逐渐降低，逆转倍数有逐渐增高的趋势。（4）10μmol/L的环孢素A和1μmol/L的雷帕霉素，开始增加乳腺癌耐药细胞株MCF-7/ADR细胞内的阿霉素浓度，并且随着浓度的增加，阿霉素浓度增加。相同浓度的药物作用下，雷帕霉素组的细胞内阿霉素浓度较环孢素A高。结论雷帕霉素能逆转MCF-7/ADR细胞株的耐药性，且效果较环孢素A好。 |
| 中文关键词:雷帕霉素 环孢素A 乳腺癌 多药耐药 逆转 |
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| Comparison About Reverse Effect of Rapamycin and Cyclosporine A on Multidrug Resistance of Human Breast Cancer Cell |
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| Abstract:ObjectiveTo compare the reverse effect of rapamycin and cyclosporine A on multidrug resistance of human breast cancer cell. MethodsThe drug resistance fold of MCF-7/ADR was detected by MTT. Human breast cancer cells MCF-7/S and MCF-7/ADR were separated to some groups respectively.In control group, ADM of different concentrations were added,and in experiment group,besides ADM of different concentrations, rapamycin or cyclosporine A of different concentrations were added,and in blank group,nothing was added. The inhibition rates were detected by MTT, then IC50 and reverse fold were calculated. Rapamycin or cyclosporine A were added to MCF-7/ADR with ADM, then concentrations of ADM in cells were detected by immunofluorescence. Results(1) The drug resistance fold of mcf-7/ADR was 12.13. (2) For MCF-7/S, the reverse fold of rapamycin and cyclosporine A were 1. (3)For MCF-7/ADR, cyclosporine A (10μmol/L) and rapamycin (1μmol/L) could decrease IC50 of ADM significantly. And as the concentration increasing, IC50 decreased, and reverse fold increased. (4) Cyclosporine A (10μmol/L) and rapamycin (1μmol/L) could increase the concentration of ADM in MCF-7/ADR cells. With the increasing concentration of drugs, the concentration of ADM in cells increased. The concentration of ADM in cells with rapamycin was higher than that with cyclosporine A of the same concentration. ConclusionRapamycin could reverse the drug resistance of MCF-7/ADR, and had better effect than cyclosporine A. |
| keywords:Rapamycin Cyclosporine A Breast cancer Multi-drug resistance Reverse |
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