| 2009年昆明地区流行的肠道病毒71型基因特征分析 |
| 投稿时间：2011-03-23 修订日期：2011-04-07 点此下载全文 |
| 引用本文：陈俊英,潘玥,李华,施海晶,赵玉娇,孙强明,马绍辉.2009年昆明地区流行的肠道病毒71型基因特征分析[J].医学研究杂志,2011,40(11):105-108 |
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| 中文摘要:目的分析2009年昆明地区肠道病毒71型分离株VP1的遗传特征。方法采用反转录-聚合酶链反应（RT-PCR）法从Vero细胞分离的病毒株基因组中扩增出VP1基因，并测序；分别用Mega4.1软件分析其遗传特征。结果中国昆明分离到的6株肠道病毒71型VP1基因的核苷酸和氨基酸同源性为95.4%~97.9%和97.3%~997%，与其他C基因型C1、C2、C3和C5的VP1的同源性为87.9%~89.1%和98.3%~99.1%,而与C4基因型的同源型为92.6%~98.5%和97.0%~997%，与SHZH98的同源性最低为92.6%和97.0%,与其他基因型的同源性为82.7%~84.6%和96.0%~97.6%。结论2009年云南昆明流行的肠道病毒71型均为C4基因型；其VP1基因保守性较好。 |
| 中文关键词:肠道病毒71型 C4基因型 VP1基因 |
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| Genetic Analysis of the VP1 Region of Human Enterovirus 71 Strains Isolated in Kunming, China, During 2009 |
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| Abstract:ObjectiveTo analyze genetic characterization of the VP1 genes of enterovirus 71 isolated in Kunming, China in 2009. MethodsSix enterovirus 71 strains were isolates in Kunming, China in 2009. By using RT-PCR, the VP1gene fragments contained 891 nucleotides in all strains were sequenced. The sequences were aligned with other enterovirus 71 sequences downloaded from GenBank using Mega4.1 software. ResultsSix isolated strains were closely related to other reference strains of subgroup C4. In VP1 gene, the homology of nucleotide and amino acid among the six isolated strains were 95.4%-97.9% and 97.3%-99.7%, respectively, and 926%-99.4% and 87.7%-100% of homology when compared with that of strains isolated from the subgroups C1,C2，C3 and C5, respectively. SHZH98 strain had less homology when compared to other strains of subgroup C4.The six strains had homology of 92.6%-98.5% and 97.0%-99.7% compared to that of other subgroups on nucleotide and amino acid, respectively. ConclusionSix strains isolated in Kunming in 2009 were belonged to subgroup C4 of enterovirus and 71.VP1 gene was more conservative. |
| keywords:Enterovirus 71 Subgroup C4 VP1 gene |
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