| 三叶青多糖抗肝损伤作用的研究 |
| 投稿时间：2011-04-17 修订日期：2011-11-22 点此下载全文 |
| 引用本文：马丹丹,李伟平,马哲龙,何良艳,蒋福升,丁志山.三叶青多糖抗肝损伤作用的研究[J].医学研究杂志,2012,41(1):33-36 |
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| 基金项目:“重大新药创制”科技重大专项“十二五”计划资助项目(2011ZX09102-011-07) |
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| 中文摘要:目的测定三叶青中多糖的含量，并研究三叶青多糖抗四氯化碳（CCl4）致急性肝损伤的作用。方法采用硫酸-苯酚法测定三叶青多糖的含量，然后将ICR小鼠随机分为5组，即空白组、模型对照组、阳性对照组，三叶青多糖高、低剂量组，分别灌胃等容积的生理盐水、联苯双酯和三叶青多糖，给药一段时间后给小鼠腹腔注射四氯化碳致其肝损伤，检测其血清中谷丙转氨酶 （ALT）、谷草转氨酶（AST）、丙二醛（MDA）及超氧化物歧化酶（SOD）水平。结果三叶青中多糖的含量为8.69%；与模型组比较，三叶青多糖治疗组 ALT、AST及MDA含量均明显降低（P<0.01），而SOD水平显著提高(P<0.01)。结论三叶青多糖对 CCl4造成的小鼠急性肝损伤具有阻抗作用。 |
| 中文关键词:三叶青 多糖 含量 肝损伤 四氯化碳 |
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| Anti-liver Damage Activity Analysis of Polysaccharide in Radix Tetrastigmatis Hemsleyani |
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| Abstract:ObjectiveTo determine the content of polysaccharide in radix tetrastigmatis hemsleyani,then to observe the effects of polysaccharide in radix tetrastigmatis hemsleyan on acute liver injury in mice.MethodsThe content of polysaccharide in radix tetrastigmatis hemsleyani was determined by phenol-sulfuric acid method.Then 40 mice of ICR species into were randomly divided 5 groups：blank control group, model group, positive control group, lowdose group and highdose group. These mice were given physiological saline,bifendate and polysaccharide in radix tetrastigmatis hemsleyani by intragastric administration every day.After a week,carbon tetrachloride was injected to cause liver injury in mice. Then serum alanine aminotransferase (ALT),aspartate aminotransferase (AST),malondialdehyde(MDA)and uperoxide dismutase(SOD) levels was determined. ResultsThe content of polysaccharide in radix tetrastigmatis hemsleyani was 8.69%.Compared with model group, ALT, AST and MDA levels were significantly reduced（P<0.01）,while SOD levels were significantly increased in treatment group of polysaccharide（P<0.01）.ConclusionPolysaccharide in radix tetrastigmatis hemsleyani has good impedance function on CCl4-induced liver injury in mice. |
| keywords:Radix tetrastigmatis hemsleyani Polysaccharide Content Liver damage Carbon tetrachloride(CCl4) |
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