| 膀胱尿路上皮癌中miRNA-300的差异表达 |
| 投稿时间：2011-01-27 修订日期：2011-03-16 点此下载全文 |
| 引用本文：徐锋,高建平,张征宇,葛京平,位志峰,程文.膀胱尿路上皮癌中miRNA-300的差异表达[J].医学研究杂志,2012,41(1):39-42 |
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| 基金项目:国家自然科学基金资助项目（30772278）；南京军区南京总医院青年课题（2009-2011） |
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| 中文摘要:目的探讨miRNA-300在膀胱尿路上皮癌中的差异表达与其生物学行为的关系。方法常规提取30例膀胱尿路上皮癌及5例正常膀胱黏膜组织中总RNA，应用荧光定量RT-PCR方法检测标本中miRNA-300的表达情况。结果与正常膀胱黏膜上皮组织相比，膀胱尿路上皮癌中miRNA-300表达明显下调（P＜0.01）。结论miRNA-300的差异表达极可能为促进膀胱尿路上皮癌的发生及发展因素之一。 |
| 中文关键词:miRNA-300 膀胱尿路上皮癌 实时荧光定量 |
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| Expression of miRNA-300 in Urothelial Carcinoma of the Bladder |
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| Abstract:ObjectiveTo investigate the gene expression disparity and its relationship with biological behavior of miRNA-300 in urothelial carcinoma of the bladder （BUC）．MethodsThirty BUC and five normal bladder mucosa tissues were collected and the total RNA was extracted routinely.The miRNA-300 expression was detected by using real-time quantitative polymerase chain reaction.ResultsThe expression of miRNA-300 was significantly down-regulated in BUC tissues than those in normal bladder mucosa. ConclusionmiRNA-300 may Participate in the pathogenesis and development of BUC． |
| keywords:miRNA-300 Urothelial carcinoma of the bladder Real-time quantitative |
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