| 抑制11β-HSD2对GC保护血管内皮细胞炎性损伤的影响 |
| 投稿时间：2011-05-30 修订日期：2011-06-01 点此下载全文 |
| 引用本文：王兴友,王丽娜,陈晓琳,陈杭薇.抑制11β-HSD2对GC保护血管内皮细胞炎性损伤的影响[J].医学研究杂志,2012,41(1):93-96 |
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| 基金项目:“十一五”军队科技攻关项目资助(08G006) |
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| 中文摘要:目的探讨阻断11β-HSD2的表达对GC保护血管内皮炎性损伤中的影响。 方法用甘草次酸（GA）抑制人脐静脉内皮细胞11β-HSD2的表达，观察GA作用后体外培养的人脐静脉内皮细胞在LPS和(或)Dex刺激下IL-6等炎性细胞因子的分泌及发生凋亡的变化，同时观察不同浓度的GA对人脐静脉内皮细胞11β-HSD2的表达的抑制情况。结果GA单独作用于人脐静脉内皮细胞时，对11β-HSD2 mRNA的表达无明显影响。但GA在HUVEC受到LPS刺激时能够明显抑制LPS诱导的11β-HSD2 mRNA的表达。此外GA与GC合用可进一步增强对11β-HSD2 mRNA的表达的抑制，GA能显著抑制LPS诱导的HUVEC分泌IL-6和sICAM-1，同时显著降低LPS诱导的HUVEC的细胞凋亡率。结论11β-HSD是GC作用的受体前调节的关键物质，GA又是传统的11β-HSD抑制剂，因此11β-HSD2完全可以作为GA抗炎等作用的1个靶点。GA通过抑制11-HSD2 mRNA的表达是其增强GC（Dex）抗炎作用的一个主要机制。GA与GC合用可进一步增强对11β-HSD2 mRNA的表达的抑制，同时加强了GC对LPS诱导的血管内皮炎性损伤的保护作用。表明GA与GC合用能够通过受体前调节机制加强抗炎效应，是一个值得关注的途径。 |
| 中文关键词:11β羟基类固醇脱氢酶2 糖皮质激素 糖皮质激素受体 血管内皮细胞 甘草次酸 |
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| Effect of GC for Protection of the Inflammatory Injury in HUVEC by Inhibiting the Expression of 11β-HSD2 |
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| Abstract:ObjectiveTo explore the effect of GC for protection of the inflammatory injury in HUVEC by inhibiting the expression of 11β-HSD2 MethodsWe used glycyrrhizic acid(GA) to inhibit the expression of 11β-HSD2，lipopolysaccharide LPS ‘injured’ endothelial cells with dexamethasone for "treatment",and then detected the expression of IL-6 and sICAM-1 and the apoptosis of HUVEC induced by LPS. ResultsHUVEC only was treated with glycyrrhizic acid(GA), the expression of 11β-HSD2 in HUVEC didn′t change.But if HUVEC was treated with LPS and GA, it could inhibit the expression of 11β-HSD2 in HUVEC,meanwhile decrease the secretion of IL-6 and sICAM-1 and the apoptosis of HUVEC induced by LPS. HUVEC being treated with both GA and Dex could achieve a powerfuler effect than only Dex.ConclusionIt indicated that GA could enhance the effects of GC by prereceptor regulation. GC attaching GA as a new treatment plan in GC should be spreaded in the future. |
| keywords:11－βHydroxysteroid dehydrogenase type2 Glucocorticoid Glucocorticoid receptor Vascular endothelial cell Glycyrrhizic acid |
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