| Topo Ⅱα在宫颈癌前病变中的表达及其与高危型人乳头瘤病毒感染的关系 |
| 投稿时间：2011-05-28 修订日期：2011-06-13 点此下载全文 |
| 引用本文：屈王蕾,高宝辉,董剑达,吕杰强.Topo Ⅱα在宫颈癌前病变中的表达及其与高危型人乳头瘤病毒感染的关系[J].医学研究杂志,2012,41(2):25-28 |
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| 基金项目:英国威康信托基金会资助项目（071845/Z/03/Z）;浙江省计划生育委员会资助项目（200930） |
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| 中文摘要:目的探讨Topo Ⅱα蛋白在宫颈癌前病变组织中的表达及其在宫颈癌发生中的作用，并探讨高危型人乳头瘤病毒感染与Topo Ⅱα表达的关系。方法选取2006年1～12月在温州医学院附属第二医院行宫颈病变诊治的患者，其中包括各级宫颈上皮内瘤变（CIN）和宫颈鳞癌（SCC）的患者。随机选取同期就诊的慢性宫颈炎患者作为对照。运用第2代杂交捕获法（HC2）检测高危型人乳头瘤病毒（HR-HPV）的表达。活体提取组织，运用免疫组织化学法（SP法）检测宫颈组织中Topo Ⅱα蛋白表达。结果Topo Ⅱα在正常宫颈上皮组织、CINⅠ、CINⅡ、CINⅢ、SCC中的表达率分别20.0%(6/30)、15.8%(6/38)、469%(15/32)、85.7%(30/35)和90.0%(27/30)，表达率逐渐增加，有统计学意义。在HR-HPV阳性患者的宫颈病灶组织中Topo Ⅱα蛋白阳性表达率高于HR-HPV阴性患者，有统计学意义。结论Topo Ⅱα蛋白表达随着宫颈癌前病变的严重程度而上调，可能参与宫颈上皮的异常增殖和恶性转变。其表达的上调与HR-HPV感染有关，可能是HR-HPV感染后导致细胞恶性转化的中间行为。 |
| 中文关键词:Topo Ⅱα 宫颈癌 人乳头瘤病毒 |
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| Expression of Topo Ⅱα in Cervical Intraepithelial Neoplasia and Its Relation to High-risk Human Papillomavirus Infection |
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| Abstract:ObjectiveTo investigate the expression of topoisomerase Ⅱα in cervical intraepithelial neoplasia (CIN) and invasive squamous cell carcinoma (SCC) and the function of cervical carcinogenesis; and study the influence of high-risk human papillomavirus (HR-HPV) infection on it. MethodsTotally 135 patients with CIN or SCC were rolled in the Second Affiliated Hospital of Wenzhou Medical College from January to December in 2006. Another 30 patients during the same period with chronic cervictits were selected as control group. Hybird capture Ⅱ assay（HC2）was used to detect the expression of HR-HPV of all the patients. All tissues were diagnosed and grouped through HE after operation. Immunohistochemical staining (SP) was used to detect the topoisomerase Ⅱα in these tissues. ResultsThe positive rate of expression of Topo Ⅱα in NCE，CINⅠ，CINⅡ，CINⅢ and SCC was 20.0%(6/30), 15.8%(6/38), 46.9%(15/32), 85.7%(30/35) and 90.0% (27/30), respectively. A progressive increase was along with the grade of the lesion (P<0.05). The positive rate of expression of TopoⅡα was increased in HR-HPV-positive lesions than that of HR-HPV-negative(64.2% vs 13.3%, P<0.05). The increased TopoⅡα expression was associated with HR-HPV (rs＝0.454,P<0.05). ConclusionTopo Ⅱα expression was up-regulated with the increasing grade of cervical lesion. TopoⅡα could be involved in abnormal proliferation and cancerization of cervix epithelium. The up-regulation of TopoⅡα was related to HR-HPV infection and could be a stage of cancerization after HR-HPV infection. |
| keywords:Topoisomerase Ⅱα Cervical cancer Human papillomavirus |
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