| 慢性乙型肝炎患者T淋巴细胞及激活亚群与基因型的分析 |
| 投稿时间：2011-04-26 修订日期：2011-04-27 点此下载全文 |
| 引用本文：章松平,王莹,陈公英.慢性乙型肝炎患者T淋巴细胞及激活亚群与基因型的分析[J].医学研究杂志,2012,41(3):65-67 |
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| 基金项目:杭州市科技发展计划（2005633Q22） |
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| 中文摘要:目的探讨慢性乙型肝炎（CHB）患者不同基因分型（B型和C型）外周血T淋巴细胞及激活亚群的表达情况。方法收集2008年11月~2010年12月杭州市第六人民医院175例CHB患者，另选取43名健康体检者作为对照。用流式细胞仪检测外周血T淋巴细胞亚群（CD3+、CD3+CD4+和CD3+CD8+）及激活亚群（CD3+HLA-DR+、CD4+HLA-DR+和CD8+HLA-DR+）的表达，荧光PCR法检测HBV基因型，化学发光法定量检测血清HBeAg含量。结果B基因型HBeAg(+)组与C基因型组间CD8细胞百分比有显著性差异，C基因型患者CD8细胞高于B基因型。各组间CD3、CD4淋巴细胞及激活亚群均无统计学意义。结论CHB患者不同基因分型与机体细胞免疫尤其是CD8细胞存在一定的关系。 |
| 中文关键词:慢性乙型肝炎 淋巴细胞 HLA-DR抗原 基因型 |
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| Correlation between Genotype and T-lymphocyte and Activated T-lymphocyte Subsets in Chronic Hepatitis B |
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| Abstract:ObjectiveTo investigate the correlation between genotype and T-lymphocyte and activated T-lymphocyte subsets in chronic hepatitis B. MethodsTotally 175 chronic hepatitis B patients and 43 healthy blood donors were enrolled in the study. The T-lymphocyte subsets(CD3+,CD3+CD4+ and CD3+CD8+)and activated T-lymphocyte subsets(CD3+HLA-DR+、CD4+HLA-DR+ and CD8+ HLA-DR+) were detected by flow cytometry.HBV genotypes was detected by a real time fluorescence PCR and HBeAg was detected by chemilumine-scence method. ResultsCompared with the B genotype HBeAg(+) group, the percentage of CD8 cells was increased in C genotype patients. While there was no difference in the percentage of CD3,CD4 cells and activated T-lymphocyte subsets with B genotype group and C genotype group.ConclusionThere was certain relation between different genotype and cellular immune in chronic hepatitis B patients, especially CD8 cells. |
| keywords:Chronic hepatitis B Lymphocyte HLA-DR antigens Genotype |
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