| 脂肪干细胞调节创面炎症反应并促进创面愈合 |
| 投稿时间：2011-06-01 修订日期：2011-07-18 点此下载全文 |
| 引用本文：董瑶,董飞君,李幼华.脂肪干细胞调节创面炎症反应并促进创面愈合[J].医学研究杂志,2012,41(3):100-103 |
| DOI：
|
| 摘要点击次数: 1321 |
| 全文下载次数: 877 |
|
|
| 中文摘要:目的利用脂肪组织来源的干细胞（adipose-derived stem cells, ADSCs）促进创面愈合并初步探讨其机制。方法采用胶原酶消化GFP转基因小鼠（GFP-C57BL）腹股沟处脂肪组织，获得ADSCs，并在体外证明其具有成骨和成脂的多向分化能力。14只C57BL小鼠随机分为两组并制作创面，实验组注射PBS缓冲液悬浮的ADSCs，对照组注射相同体积的PBS缓冲液。观察并分析创面愈合情况，通过荧光显微镜观察移植细胞的存活情况，利用real-time PCR检测创面组织中促炎因子白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、单核细胞趋化蛋白-1（TCP-1）以及抗炎因子白细胞介素-10（IL-10）的表达。结果分离的细胞具有较强的增殖能力，并具有多向分化潜能。同种异体移植ADSCs可以显著促进创面的愈合速度。移植两周后，荧光显微镜观察创面区域有少量ADSCs存活。与对照组相比，实验组小鼠的创面组织中促炎因子IL-1、IL-6和TCP-1表达下降，抗炎因子IL-10表达上升。结论通过同种异体局部注射ADSCs可以显著促进皮肤创面的愈合，其机制可能与ADSCs降低了创面局部的炎症反应相关 |
| 中文关键词:脂肪干细胞 皮肤创伤愈合 炎症 |
| |
| Adipose-derived Stem Cells Modulate Inflammation During Wound Healing and Enhance Wound Repair |
|
|
| Abstract:ObjectiveTo investigate the feasibility of adipose derived stem cells (ADSCs) from GFP-C57BL mouse for wound repair and explore the mechanism. MethodsADSCs were isolated from groin adipose tissue of GFP-C57BL mice. The differentiation potential of ADSCs was investigated. Fourteen C57BL mice were randomly divided into two groups:experimental group was treated with ADSCs suspended in PBS, and control group was treated with the same volume of PBS without ADSCs. Wound repair and the survival of ADSCs were observed and analyzed. Real-time PCR was used to investigate the effect of ADSCs on inflammation during wound repair. ResultsThe cultivated ADSCs had the ability to proliferate continuously and showed characteristics of multi-potent mesenchymal stem cells. Allogeneic transplantation of ADSCs significantly enhanced wound repair. After two weeks of transplantation, a small number of ADSCs were observed using a fluorescence microscope. ADSCs inhibited the RNA expression of interleukin-1 (IL-1), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (TCP-1) and promoted the RNA expression of IL-10 in wound area. ConclusionAllogeneic transplantation of ADSCs could enhance wound repair, which may be due to their regulation effect on inflammation during wound repair. |
| keywords:Adipose tissue derived stem cells Skin wound healing Inflammation |
| 查看全文 查看/发表评论 下载PDF阅读器 |
