| 淋病奈瑟菌gyrA和parC基因喹诺酮耐药决定区突变与环丙沙星耐药相关性研究 |
| 投稿时间：2011-05-30 修订日期：2011-06-16 点此下载全文 |
| 引用本文：高家良,王黎芳,李洵璐,周娇萍,孙爱华.淋病奈瑟菌gyrA和parC基因喹诺酮耐药决定区突变与环丙沙星耐药相关性研究[J].医学研究杂志,2012,41(3):107-110 |
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| 基金项目:浙江省医药卫生科学研究基金资助项目（2004A018） |
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| 中文摘要:目的分析宁波地区淋病奈瑟菌临床菌株gyrA和parC基因喹诺酮耐药决定区（QRDR）突变类型及与喹诺酮类药物耐药的相关性。方法采用琼脂稀释法检测137株淋病奈瑟菌临床菌株对6种抗生素的最小抑菌浓度（MIC），PCR法扩增50株环丙沙星耐药的淋病奈瑟菌临床菌株gyrA 和parC基因喹诺酮耐药决定区，并进行测序分析。结果137株淋病奈瑟菌临床菌株对大观霉素、头孢曲松、四环素、青霉素、氧氟沙星和环丙沙星的耐药率分别为0、0、75.2%、76.6%、97.1%和100%。50株临床菌株测序发现，gyrA基因存在3种核苷酸序列发生错义突变，其中S91F突变发生在所有检测菌株，49株D95位发生突变；parC基因突变位点较多且相对比较分散，其中28株parC基因85、86、87、88和91位发生单位点突变，9株parC基因发生双重突变。结论青霉素、四环素、氧氟沙星和环丙沙星已不能作为治疗淋病的常规用药，大观霉素和头孢曲松仍可推荐用药。gyrA和parC基因突变在淋病奈瑟菌对喹诺酮类药物耐药中起重要作用。 |
| 中文关键词:淋病奈瑟菌 gyrA和parC基因 突变 序列分析 耐药性 |
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| Correlation of Mutation Patterns in gyrA and parC Genes in Neisseria gonorrhoeae Isolates with Quinolone Resistance |
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| Abstract:ObjectiveTo analyze mutations patterns in the quinolone-resistance-determining-region (QRDR) in gyrA and parC genes and stndy the correlation with quinolone resistance in Neisseria gonorrhoeae isolates in Ningbo. MethodsThe MICs of 6 antimicrobials were determined by agar dilution method. PCR and DNA sequencing were conducted to analyze QRDR associated genes of gyrA and parC in 50 isolates. ResultsThe resistance rate of the 137 strains were 0, 0, 75.2%, 76.6%, 97.1% and 100% to spectinomycin, cefriaxone, tetracycline, penicillin, ofloxacin and ciprofloxacin respectively. Sequencing analysis of gyrA revealed that all 50 isolates had mutation of S91F and 49 resistant isolates had mutation of D95G/A/N/E. Mutations in parC gene were at 85, 86, 87 and 91.And 37 resistant isolates had at least one mutation in QRDR within parC, in which 28 isolates had single mutation, and 9 isolates had double mutations in parC. ConclusionTetracycline, penicillin, ofloxacin and ciprofloxacin should not be routine therapy for gonorrhoea, while the use of spectinomycin and cefriaxone is recommended. Mutations in gyrA and parC genes play an im portant role in the development of quinolone resistance in N. gonorrhoeae. |
| keywords:Neisseria gonorrhoeae gyrA and parC genes Mutation Sequence analysis Drug resistance |
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