| RhIL-21对NK-92MI细胞生物学活性的影响 |
| 投稿时间：2011-05-20 修订日期：2011-07-07 点此下载全文 |
| 引用本文：郑瑞,陈葆国,颜卫华,李伯利,章卫国,干灵红.RhIL-21对NK-92MI细胞生物学活性的影响[J].医学研究杂志,2012,41(3):120-124 |
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| 中文摘要:目的探讨rhIL-21对NK-92MI细胞免疫功能的影响。方法以不同浓度（25～100ng/ml）的rhIL-21培养NK-92MI细胞24～72h，观察NK-92MI细胞的增殖。以50ng/ml的rhIL-21处理NK-92MI不同时间后， FACS检测NK-92MI细胞的凋亡，NK-92MI细胞表面NKG2D受体及胞内颗粒酶-B蛋白的表达；细胞杀伤试剂盒检测NK-92MI 细胞对靶细胞K-562的细胞毒效应；RT-PCR检测NK-92MI受体NKG2D，效应分子颗粒酶-B、穿孔素及细胞因子IFN-γ基因mRNA的表达；ELISA检测其分泌IFN-γ的水平。结果RhIL-21虽能抑制NK-92 MI 细胞的增殖（P＜0.05），但不会促进细胞的凋亡（P＜0.05）。RhIL-21能促进NK-92MI 细胞穿孔素、IFN-γ、颗粒酶-B、NKG2D受体基因及相应蛋白质的表达上调（P＜005）。rhIL-21预处理组NK-92MI对K-562细胞的细胞毒效应也明显增强（P＜0.05）。结论RhIL-21能增强NK-92 MI细胞的细胞毒效应，IL-21在肿瘤的免疫治疗中有潜在临床应用价值。 |
| 中文关键词:重组人白细胞介素-21 NK-92MI细胞 干扰素-γ NKG2D 颗粒酶 穿孔素 |
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| Effect of rhIL-21 on NK-92MI Cells |
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| Abstract:ObjectiveTo investigate the effect of rhIL-21 on NK-92MI cells. MethodsUsing in vitro cultural experiments, NK-92MI cells were treated with different concentration of rhIL-21 and then the proliferation were tested at different time. In addition, after NK-92MI cells treated with 50ng/ml rhIL-21 for 48h or 72h，the NK-92MI cell activity, apoptotic cells, the expressions of NKG2D and granzyme-B was measured using FACS assay.The levels of IFN-γfrom the supernatants were detected by ELISA. The expressions of NKG2D, perforin, granzyme-B, and IFN-γ mRNA were determined by RT-PCR. ResultsRhIL-21 inhibited NK-92MI cell proliferation.However,rhIL-21 did not affect cell apoptosis.RhIL-21 increased the expressions of the effector molecules granzyme-B and IFN-γ as well as activation receptor NKG2D at the mRNA and protein levels.Furthermore, compared with control, rhIL-21 enhanced the cytotoxicity of NK92-MI cells against K-562 target. ConclusionRhIL-21 could enhance the cytotoxicity of NK92-MI cells possibly by up-regulating the expression of activation and effector molecular, suggesting that IL-21 might have therapeutically effect to enhance NK cell-mediated antitumour responses. |
| keywords:Recombination human interleukin 21(rhIL-21) NK-92MI cells Interferon-γ(IFN-γ) NKG2D Granzyme-B Perforin |
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