| PARP-1基因多态性与临夏回族自治州回族人群胃癌易感性的关联研究 |
| 投稿时间：2011-04-27 修订日期：2011-06-03 点此下载全文 |
| 引用本文：唐小凡,何正德,李玉民,蒋祥林.PARP-1基因多态性与临夏回族自治州回族人群胃癌易感性的关联研究[J].医学研究杂志,2012,41(3):151-154 |
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| 中文摘要:目的探讨聚腺苷二磷酸核糖聚合酶-1（PARP-1）Val762Ala的单核苷酸多态与临夏回族自治州回族人群胃癌易感性的关系。方法采用病例-对照研究设计，研究对象为临夏回族自治州居民（20岁以上，居住满20年以上，3代无族外通婚）。分两组：胃癌组200例，均经组织学确诊。对照组210例，按年龄、性别进行匹配的无肿瘤史的健康回族志愿者。抽提血液DNA，采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)进行基因分型。使用ELISA（enzyme-linkedimmunosorbent assay）法检测血浆中H.pylori的IgG抗体，以检测研究对象是否有H.pylori感染。用SPSS 13.0统计软件分析试验结果。结果PARP-1 762Val/Ala+Ala/Ala在胃癌组显著增高（OR：2.158，95% CI：1.404～3.315，P=0.020）。交互作用分析显示：家族史阳性的Val/Ala+ Ala/Ala基因型患胃癌的风险是家族史阴性的Val/Val 基因型的4.125倍（OR：4.125，95% CI：2.117～8.038，P=0.000）；食腌菜的Val/Ala+ Ala/Ala基因型患胃癌的风险是不食腌菜的Val/Val基因型的3.814倍（OR：3.814，95% CI：2150～6.766，P=0.000）；H.pylori感染阳性的Val/Ala+ Ala/Ala基因型患胃癌的风险是H.pylori感染阴性Val/Val基因型的4.085倍（OR：4.085，95% CI：2.197～7.597，P=0.000）。结论PARP-1 Val762Ala单核苷酸多态与临夏回族自治州回族人群胃癌易感性增高相关。PARP-1 Val/Ala+Ala/Ala基因型分别与家族史、食腌菜、H.pylori感染在胃癌的发病风险中存在着加乘交互效应。 |
| 中文关键词:胃癌 聚腺苷二磷酸核糖聚合酶-1 基因多态性 |
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| Correlation Between the Nucleotide Polymorphisms of PARP-1 and the Susceptibility of Gastric Cancer in Hui Ethnic Group of Linxia Hui Autonomous Prefecture |
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| Abstract:ObjectiveTo explore the correlation between nucleotide polymorphisms in polymerase-1（PARP-1）Val762Ala and the susceptibility of gastric cancer in Hui ethnic group of Linxia Hui Autonomous Prefecture.MethodsA case-control study was performed.All investigated objects were Hui ethnic population of Linxia Hui Autonomous Prefecture that included 2 groups: gastric cancer group（200 gastric cancer patients），and control group （210 controls, frequency-matched for age and sex）. Polymerase chain reaction-restrieted fragments length polymorphism(PCR-RFLP) method was used to detect genotypes of PARP-1Val762Ala polymorphisms. ELASA（enzyme-linked immunosorbent assay） method was used to identify IgG to H.pylori.The results were analyzed by SPSS13.0.ResultsPARP-1762Val/Ala+Ala/Ala genotypes were overrepresented in the gastric cancer group （OR：2.158，95%CI：1.404-3315,P=0.020）. Interaction analysis showed that: Val/Ala+Ala/Ala genotypes carriers with positive family history of gastric cancer had 4.125 fold increased risk for developing gastric cancer compared with Val/ Val genotypes carriers with negative family history of gastric cancer （OR：4.125，95%CI：2.117-8.038，P=0.000）；Val/Ala+ Ala /Ala genotypes carriers with eating pickle vegetables had 3814 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with not-eating pickle vegetables（OR：3.814，95%CI：2.150-6.766，P=0.000）. Val/Ala+ Ala/Ala genotypes carriers with positive H.pylori infection had 4.085 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with negative H.pylori infection（OR：4.085，95%CI：2.197-7.597,P=0.000）. ConclusionPARP-1Val762Ala contributed to the increased susceptibility of gastric cancer in Hui ethnic group in Linxia Hui Autonomous Prefecture. Multiplicative interactions lied in between PARP-1762Val/Ala+Ala/Ala genotypes and positive family history of gastric cancer, eating pickle vegetables, H.pylori infection in risk of developing gastric cancer. |
| keywords:Gastric cancer Poly(ADP-ribose)polymerase-1 Polymorphism |
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