| Notch2、Notch4受体在哮喘小鼠肺中的表达及布地奈德、姜黄素对其影响 |
| 投稿时间：2011-08-22 修订日期：2011-09-05 点此下载全文 |
| 引用本文：崇蕾,张维溪,聂颖,王婷,李昌崇.Notch2、Notch4受体在哮喘小鼠肺中的表达及布地奈德、姜黄素对其影响[J].医学研究杂志,2012,41(4):73-75 |
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| 基金项目:浙江省自然科学基金资助项目（Y2090327） |
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| 中文摘要:目的探讨Notch2、Notch4受体在哮喘小鼠肺组织中的表达水平以及布地奈德和姜黄素对其的影响。 方法将32只Balb/c小鼠按随机数字表法随机分为正常对照组（A组），哮喘组（B组），布地奈德组（C组）和姜黄素组（D组），每组8只。以卵白蛋白致敏激发建立哮喘小鼠气道炎症模型，每组小鼠分别于末次激发后处死，取肺组织行HE染色观察炎症浸润程度，免疫组织化学染色和RT-PCR法检测各组小鼠肺组织中Notch2和Notch4受体蛋白和mRNA的表达水平。 结果(1)C、D组Notch2蛋白及mRNA表达［分别为（0.075±0.002）、（0.073±0.002）和（0.088±0.012）、（0.085±0.008）］均明显低于B组［分别为（0.104±0.005）、（0.275±0.015）］，但仍高于A组［分别为（0.049±0.004）、（0.041±0.007）］，P均＜0.05。 (2)4组Notch4蛋白及mRNA表达分别为（0.121±0.004）、（0.118±0.003）、（0.117±0.004）和（0.117±0.005）以及（0.595±0.019）、（0.601±0.125）、（0.606±0.145）、（0.604±0.019），差异无统计学意义，均P＞0.05。 结论Notch2受体参与哮喘小鼠气道炎症过程，布地奈德和姜黄素可能部分通过Notch2受体对哮喘小鼠气道炎症有一定抑制作用；Notch4受体对哮喘小鼠气道炎症无明显影响。 |
| 中文关键词:Notch受体 哮喘气道炎症 布地奈德 姜黄素 |
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| Expression of Notch2,Notch4 Receptors in Lung of Asthmatic Mice and the Effects of Budesonide and Curcumin |
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| Abstract:ObjectiveTo observe the expressions of Notch2,Notch4 receptors in the lung and the effects of budesonide and curcumin on the airway inflammation in asmatic mice. Methods32 Balb/c mice were randomly divided into 4 groups：normal control group(A group),asthma group(B group),budesonide group(C group) and curcumin group (D group),with each 8 mice.We use ovalbumin to allergize and challenge mice to establish asthmatic airway inflammation model.After the last challenge,each mouse was killed and its lung tissues were extracted for HE staining,immunohistostaining and RT-PCR.The airway inflammation invasion degree was observed through HE staining,and Notch2,Notch4 receptor proteins and mRNA expression levels were analyzed through immunohistostaining and RT-PCR respectively. Results①Notch2 receptor protein and mRNA expression level in C and D groups ［（0.075±0.002）,（0.073±0.002）and（0.088±0.012）,（0.085±0.008） respectively］were significantly lower than B group［（0.104±0.005）,（0.275±0.015）respectively］,but still higher than A group［（0.049±0.004）,（0.041±0.007）respectively］,with all P＜0.05. ② Notch4 receptor protein and mRNA expression level in the four groups［(0.121±0.004),(0.118±0.003),(0.117±0.004),(0.117±0.005) and （0.595±0.019）,（0.601±0.125）,（0.606±0.145）,（0.604±0.019） respectively］ had no significant differences, with all P＞0.05.ConclusionNotch2 receptor takes part in the airway inflammation process of asthmatic mice,and budenoside and curcumin may inhibit asthmatic mice airway inflammation partly through Notch2 receptor.Notch4 receptor has no effect on the asthmatic mice airway inflammation. |
| keywords:Notch receptor Asthmatic airway inflammation Budesonide CurcuminNotch |
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