| nox5表达异常对缺血性心肌病患者循环EPCs功能的影响 |
| 投稿时间：2011-07-12 修订日期：2011-07-27 点此下载全文 |
| 引用本文：李虹,刘强,白小涓,王宁夫.nox5表达异常对缺血性心肌病患者循环EPCs功能的影响[J].医学研究杂志,2012,41(4):107-111 |
| DOI：
|
| 摘要点击次数: 1065 |
| 全文下载次数: 991 |
|
|
| 中文摘要:目的探查缺血性心肌病患者循环内皮祖细胞（endothelial progenitor cells，EPCs）功能是否异常，了解活性氧（reactive oxygen species，ROS）和ROS生成的相关基因nox5表达对EPCs功能的影响。方法入选缺血性心肌病患者及健康对照组各7例，测定外周血EPCs数量、增殖、迁移、成血管能力的改变，检测nox5蛋白及mRNA的表达，进一步沉默nox5表达，检测对EPCs功能及ROS产量的影响。结果与健康对照组比较，缺血性心肌病患者循环EPCs数目、增殖、迁移能力等未见异常；但体外培养过程中内皮系表面标志KDR的表达减少，体外成血管能力下降，伴随nox5蛋白及mRNA表达增加、活性氧生成增加；沉默nox5表达可降低细胞内ROS产量，改善EPCs成血管能力。结论本研究提示，缺血性心肌病患者循环EPCs体外成血管能力异常，细胞内ROS生成增加，ROS生成的相关基因nox5蛋白及mRNA表达上调；沉默nox5表达可降低细胞内ROS产量并改善EPCs功能。 |
| 中文关键词:内皮祖细胞 nox5 缺血性心肌病 活性氧 |
| |
| Effects of Nox5 Expression Disorders on Circulatory Endothelial Progenitor Cells Ability in Patients with Ischemic Cardiomyopathy |
|
|
| Abstract:ObjectiveTo detect if circulatory endothelial progenitor cells (EPCs) of ischemic cardiomyopathy(ICM) were in an anomalous position, and to find if the mRNA and protein expression of nox5, as a irreplaceable gene that related to the production of reactive oxygen species (ROS), has effects on EPCs function. Methods7 patients of ICM and 7 heatlhy people as control, were taken peripheral venous blood to isolate mononuclear cells, then CD34＋/KDR＋ cells were counted. Proliferation, migration and vascularization of EPCs cultured in vitro were detected, together with protein and mRNA expression of nox5. Further studies were done to investigate if silencing nox5 gene expression would improve EPCs function and decrease ROS produnction. ResultsThere was no significant difference in quantity, proliferation and migraton between ICM group and control. KDR expression and vascularization ability declined in ICM group, accompanied by increasing gene expression of nox5 and ROS production. Silencing nox5 gene expression decreased ROS produnction and improved EPCs vascularization ability of ICM patients. ConclusionThe study found circulatory EPCs of ICM patients may have dysdifferentiation to endothelial cells, togethered with protein exprossion of nox5 increased and ROS production elevated.Sliencing nox5 gene expression decreased ROS produntion and made EPCs vascularization ability better. |
| keywords:Endothelial progenitor cells Nox5 Ischemic cardiomyopathy Reactive oxygen species |
| 查看全文 查看/发表评论 下载PDF阅读器 |
