| CXCR4和MMP-9在胃癌组织中表达及其与淋巴结转移的关系 |
| 投稿时间：2011-08-16 修订日期：2011-09-05 点此下载全文 |
| 引用本文：徐斌,韩绍伟,周笑珍,李立义,蒋平.CXCR4和MMP-9在胃癌组织中表达及其与淋巴结转移的关系[J].医学研究杂志,2012,41(4):112-115 |
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| 基金项目:温州市科技计划基金资助项目（Y20090061）;温州市瓯海区科技计划资助项目（20090137） |
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| 中文摘要:目的探讨胃癌组织中CXCR4和MMP-9的表达与淋巴转移的关系及两者的相关性。方法S-P免疫组化法及反转录-聚合酶链反应(RT-PCR)检测CXCR4和MMP-9在30例无淋巴转移与46例有淋巴转移人胃癌组织中的表达水平。结果CXCR4蛋白在胃癌组织中的阳性表达率为55.26%，其中淋巴结转移组阳性表达率分别为73.91%，无淋巴转移组阳性表达率为36.37%，差异有统计学意义（P=0.000）。MMP-9蛋白在胃癌组织中阳性表达率（67.11%），其中淋巴转移组阳性表达率为80.43%，无淋巴结转移者和46.67%，差异有统计学意义(P=0.002)。CXCR4和MMP-9蛋白及mRNA在胃癌中的阳性表达随淋巴结转移增加而增加，且CXCR4阳性表达与VEGF-C阳性表达呈显著正相关(r=0.498，P<0.05)。结论胃癌CXCR4的表达和MMP-9的表达与胃癌淋巴结转移呈正相关，而且两者之间存在一定相关性，联合可能对预测胃癌患者淋巴结转移及判断预后有重要临床价值。 |
| 中文关键词:胃癌 CXCR4 MMP-9 淋巴转移 |
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| Correlation of CXCR4 and MMP-9 Expressions with Lymph Node Metastasis in Gastric Cancer |
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| Abstract:ObjectiveTo investigate the correlation of CXCR4 and MMP-9 expressions with lymph node metastasis in gastric cancer. MethodsA total of 30 cases of gastric cancer without lymphatic metastasis and 46 cases of gastric cancer with lymphatic metastasis were enrolled in this study. Immunohistochemical staining and RT-PCR were performed to detect the expression of CXCR4 and MMP-9 in gastric cancer. ResultsThe total positive rate of CXCR4 expression in these samples was 55.26%. It was higher in these cases with lymph node metastasis than that of patients without lymph node metastasis (73.91% vs 36.37%, P=0.000). Meanwhile, the total positive rate of MMP-9 expression in these samples was 67.11%. And the positive rate with lymph node metastasis was higher than that of patients without lymph node metastasis (80.43% vs 46.67%, P=0.002). Furthermore, the more lymphatic metastasis, the higher positive expression of CXCR4 and MMP-9 it was. The expression level of CXCR4 and MMP-9 were consistent with each other (r=0406,P<0.01). ConclusionExpressions of CXCR4 and MMP-9 are closely related to the lymphatic metastasis of gastric cancer, which may provide an evidence for diagnosis and assessment of prognosis in gastric cancer. |
| keywords:Gastric cancer CXCR 4 MMP - 9 Lymphatic metastasis |
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