| CMIA、FPIA及MS在全血环孢霉素A浓度监测中的对比分析 |
| 投稿时间：2011-07-06 修订日期：2011-07-27 点此下载全文 |
| 引用本文：谢服役,王峰,吴巧萍.CMIA、FPIA及MS在全血环孢霉素A浓度监测中的对比分析[J].医学研究杂志,2012,41(4):118-120 |
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| 中文摘要:目的通过荧光偏振免疫法(FPIA)、化学发光微粒子法（CMIA）和质谱法（MS）对全血环孢霉素A(CsA)谷浓度测定结果的比较，了解3种方法检测结果的差异，以便更加合理地选择CsA浓度监测方式。方法采集分离100例肾移植术后患者稳态浓度的血浆样品，以CMIA、FPIA以及MS法测定CsA浓度，并对3种方法测定结果进行统计分析。 结果FPIA的线性范围在0~800ng/ml，最低检测浓度为43.0ng/ml，而CMIA法测定的线性范围在0~1500ng/ml，最低检测浓度为24.1ng/ml；3种方法中，FPIA法和CMIA法的检测结果与和MS的相关系数r分别为0.96和0.97；FPIA和CMIA法的检测结果均高于MS（P<001），但相比FPIA法，CMIA的测定值明显更低，两组数据差异具有统计学意义（P<0.01）； Bland-Alman一致性分析显示，CMIA法的测定结果较FPIA法普遍偏低，平均偏差为-76.5ng/ml。结论CMIA相比FPIA法，灵敏度和特异度更高，是更为客观和理想的CsA血药浓度的检测方法，建议可推广入临床常规监测。 |
| 中文关键词:环孢霉素A 荧光偏振免疫法(FPIA) 化学发光微粒子法（CMIA） 质谱法（MS） |
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| Comparison of FPIA, CMIA or MS for Monitoring Concentration of CsA in Human Whole Blood |
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| Abstract:ObjectiveTo compare fluorescence polarization immunoassay (FPIA), chemiluminescent microparticle immunoassay (CMIA) or mass spectrometry (MS) for detecting concentration of CsA in whole blood in order to choose a more rational way for clinical drug monitoring. MethodsWe collected 100 cases of steady-state plasma samples and tested concentration of CsA by FPIA, CMIA or MS, then processed the obtained data by statistical analysis. ResultsLinear detection range of FPIA was 0-800ng/ml and the minimum detectable concentration was 43.0ng/ml, and the linear range of CMIA method was 0-1500ng/ml, with minimum detectable concentration at 24.1ng/ml. Correlation coefficient between FPIA or CMIA and MS was 0.96 and 0.97, respectively. One-way ANOVA analysis showed results obtained from FPIA and CMIA were higher than those from MS, while there was still significant difference between FPIA and CIAM (P<0.01). Bland-Alman consistency analysis manifested CsA concentration tested by CMIA was generally lower, with an mean deviation of -76.5ng/ml. ConclusionCompared to FPIA, CMIA is more sensitive and specific, therefore is a more objective and desirable method for clinical drug monitoring of CsA. |
| keywords:Cyclosprin A Fluorescence polarization immunoassay (FPIA) Chemiluminescent microparticle immunoassay (CMIA) Mass spectrometry (MS) |
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