| 表面活性蛋白A不同亚型在脂多糖诱导肾小管上皮细胞IL-8表达中的作用 |
| 投稿时间：2014-06-13 修订日期：2014-06-27 点此下载全文 |
| 引用本文：胡凤琪,崔龙,明静,袁海.表面活性蛋白A不同亚型在脂多糖诱导肾小管上皮细胞IL-8表达中的作用[J].医学研究杂志,2015,44(1):51-53 |
| DOI：
10.3969/j.issn.1673-548X.2015.01.015 |
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| 基金项目:国家自然科学基金资助项目(81300617) |
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| 中文摘要:目的 探讨表面活性蛋白A(surfactant protein A,SP-A)不同亚型(SP-A1,SP-A2)对脂多糖(lipopolysaccharide,LPS)诱导的人近曲肾小管上皮细胞(HK-2)IL-8表达的影响。方法 体外培养HK-2细胞,分别转染SP-A1、SP-A2及阴性对照siRNA,实时定量PCR检测SP-A1及SP-A2 mRNA表达水平,并给予1μg/ml LPS刺激8h,ELISA观察转染不同siRNA后IL-8蛋白表达变化。结果 转染SP-A1、SP-A2 siRNA可分别显著下调转染SP-A1、SP-A2的mRNA表达水平(P<0.05);1μg/ml LPS刺激8h可引起HK-2细胞IL-8蛋白表达显著增加(P<0.05),低表达SP-A2 可下调LPS诱导的IL-8表达(P<0.05)。结论 LPS刺激下肾小管上皮细胞IL-8表达增加与SP-A2的表达有关,SP-A2可能在肾脏炎性反应中发挥重要作用。 |
| 中文关键词:脂多糖 肾小管上皮细胞 表面活性蛋白A 白介素-8 |
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| Effects of Surfactant Protein A1 and A2 on the Expression of IL-8 in Human Renal Proximal Tubular Epithelial Cells Stimulated by Lipopolysaccharide |
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| Abstract:Objective To investigate the effects of SP-A1 and SP-A2 on the expression of IL-8 in human renal proximal tubular epithelial cells (HK-2) stimulated by 1ipopolysaccharide (LPS). Methods HK-2 cells were transfected with SP-A1 and SP-A2 siRNA and treated with 1μg/ml LPS for 8h. Expressions of SP-A1 and SP-A2 was detected by RT-PCR. Expression of IL-8 was determined by ELISA. Results Transfected SP-A1 or SP-A2 siRNA significantly decreased the mRNA levels of SP-A1 or SP-A2. The level of IL-8 in HK-2 cells was significant increased after treatment with LPS. Low expressed SP-A2 siRNA Ldownregulated IL-8 expression induced by LPS in HK-2 cells. Conclusion SP-A2 contributes to LPS induced IL-8 expression. SP-A2 may play an important role in the modulation of renal inflammation. |
| keywords:Lipopolysaccharide Renal tubular epithelial cells Surfactant protein A IL-8 |
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