| 中国汉族人群apoBVNTR等位基因的多态性分析及其分型标准物的克隆制备 |
| 投稿时间：2014-05-04 修订日期：2014-06-14 点此下载全文 |
| 引用本文：骆晓枫,洪莹芬,何江,单翼龙,黎扬斯,林建勋,谢金卫,周俊宜,周俊梅.中国汉族人群apoBVNTR等位基因的多态性分析及其分型标准物的克隆制备[J].医学研究杂志,2015,44(1):83-87 |
| DOI：
10.3969/j.issn.1673-548X.2015.01.024 |
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| 中文摘要:目的 对中国汉族人群的apoB VNTR等位基因进行分布频率、序列结构等多态性分析,并通过分子克隆技术制备apoB VNTR等位基因分型标准物。方法 通过PCR扩增并结合电泳、测序等方法分析人群apoB VNTR等位基因的多态性,并将该人群中得到的所有类型等位基因片段插入pUC 重组质粒, 经转染、扩大培养、扩增及再鉴定后, 制备出apoB VNTR等位基因分型标准物。结果 所检测人群中共检测出16种apoB VNTR等位基因,发现核心序列存在变异体,并成功制备出分型标准物。结论 对apoB VNTR的多态性分析应结合等位基因的分布频率与核心序列的结构,所制备的apoB VNTR等位基因分型标准物使apoB VNTR电泳法分型更加准确。 |
| 中文关键词:apoB VNTR 遗传多态性 等位基因分型标准物 |
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| Analysis of ApoB VNTR Polymorphism in Han Nationality and Construction of Standard apoB VNTR Allelic Ladder via Molecular Cloning |
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| Abstract:Objective To analyze apoB VNTR polymorphism including genotype frequency and sequence structure,and to constructe the apoB VNTR allelic ladder by molecular cloning. Methods PCR,gel electrophoresis and sequencing were used in analyzing apoB VNTR polymorphism. Allelic fragments of apoB VNTR were inserted into pUC plasmids. After cloning the allelic fragment, the recombinant plasmids were sequenced. Then we denominated them and used them as template for re-amplification to generate the apoB VNTR standard ladder. Results Totally 16 alleles of apoB VNTR were found in the group. Some variants of core sequence were found. The authors succeeded in producing large quantity of standard allelic ladder of apoB VNTR. Conclusion Genotype frequency and sequence structure should be combined in the Analysis of apoB VNTR polymorphism. ApoB VNTR allelic ladder is of high value for apoB VNTR typing by electrophoresis. |
| keywords:ApoB VNTR Genetic polymorphism Allelic ladder |
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