| 细胞外基质对哮喘模型大鼠气道平滑肌细胞免疫调节作用 |
| 投稿时间：2014-06-20 修订日期：2014-07-07 点此下载全文 |
| 引用本文：陈忠仁,梁梅兰.细胞外基质对哮喘模型大鼠气道平滑肌细胞免疫调节作用[J].医学研究杂志,2015,44(1):113-116 |
| DOI：
10.3969/j.issn.1673-548X.2015.01.033 |
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| 中文摘要:目的 研究在哮喘状态下,细胞外基质(extracellular matrix,ECM)对气道平滑肌细胞(airway smooth muscle cells,ASMCs)的免疫调节作用。方法 健康雄性Wistar大鼠随机分为哮喘组(8只)及正常对照组(8只)。大鼠哮喘模型使用卵清蛋白(OVA)致敏及激发的方法建立。分离培养得到的大鼠ASMCs分别置于包被有LA及COL-1的细胞瓶中进行培养。结果 Western blot法检测结果显示,eotaxin、TGF-β1的表达,哮喘空白组高于正常对照空白组(P < 0.05),哮喘组内ECM的干预促进表达的增加(P < 0.05),正常对照组表达差异无统计学意义(P > 0.05)。结论 ECM影响哮喘ASMCs的免疫功能。 |
| 中文关键词:哮喘 细胞外基质 气道平滑肌细胞 免疫调节 |
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| Regulation of Extracellular Matrix in the Immunity of Airway Smooth Muscle Cell in Asthmatic Rat |
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| Abstract:Objective To study extracellular matrix (ECM) in airway smooth muscle cells (ASMCs) immunomodulatory effects in asthma status. Methods Healthy male Wistar rats were randomly divided into asthmatic and control groups with eight in each group. Asthmatic rat model was established in using ovalbumin (OVA) sensitization and stimulation methods. ASMCs were isolated and cultured in the flasks, which coated with LA and COL-1. Expression of eotaxin and TGF-β1 protein in ASMCs was detected by Western blot. Results Western blot showed that the expression level of eotaxin,TGF-β1 among the different groups was higher in Asthma group than in the normal control group (P < 0.05). Interventions ECM increased expression within asthma group (P < 0.05). No difference was found in expression in control group (P > 0.05). Conclusion ECM can affect the immune function in asthmatic ASMCs. |
| keywords:Asthma Extracellular matrix Airway smooth muscle cell Immunity |
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