| γ-生育三烯酚与5-氟尿嘧啶联用对人胃癌细胞SGC-7901的增殖抑制作用 |
| 投稿时间：2014-08-14 修订日期：2014-09-01 点此下载全文 |
| 引用本文：姜佳佳,孙文广,屠越华,阎雅更,董凤丽,葛声.γ-生育三烯酚与5-氟尿嘧啶联用对人胃癌细胞SGC-7901的增殖抑制作用[J].医学研究杂志,2015,44(2):59-62 |
| DOI：
10.3969/j.issn.1673-548X.2015.02.016 |
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| 基金项目:国家自然科学基金资助项目(面上项目)(81273061) |
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| 中文摘要:目的 离体条件下研究γ-生育三烯酚(γ-tocotrienol, γ-T3)与5-氟尿嘧啶(5-FU)联合应用对人胃癌细胞SGC-7901增殖抑制的作用方式。方法 采用CCK-8法用不同浓度5-FU(0、30、50、70、90、110μmol/L)、不同浓度γ-T3(0、5、10、20、30、40μmol/L)以及不同浓度5-FU(0、30、50、70、90、110μmol/L)联合γ-T3(20或30μmol/L)分别作用于对数生长期的SGC-7901细胞48h, 测定各药物对SGC-7901细胞增殖抑制率, 并通过两药相互作用系数(coefficient of drug interaction, CDI)值[1]判定5-FU和γ-T3联合作用效果。用Annexin Ⅴ-FITC/PI双染的流式细胞术法分别检测5-FU(30μmol/L)和γ-T3(20μmol/L)单独应用及两药联合应用48h诱导SGC-7901细胞凋亡情况。结果 5-FU和γ-T3均可抑制SGC-7901细胞的增殖, 并随着浓度的增加抑制作用增强(P < 0.05), 增殖抑制作用呈明显的剂量效应关系;而联合应用与对照组和单药组相比对SGC-7901细胞的增殖抑制作用增强, 有增效作用, 其差异有统计学意义(P < 0.05);流式细胞术凋亡结果表明, 5-FU和γ-T3均可诱导SGC-7901细胞发生凋亡的作用(P < 0.05), 5-FU和γ-T3联合较单独应用, 诱导细胞凋亡的作用增强(P < 0.05)。结论 γ-T3与5-FU联合用药与单独用药相比对人胃癌细胞SGC-7901的增殖抑制及诱导凋亡有明显的增效作用。 |
| 中文关键词:SGC-7901 γ-T3 5-FU 联合用药 |
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| Antiproliferative Effect of Combined Treatment with γ-Tocotrienol and 5-FU on Gastric Cancer SGC-7901 Cells |
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| Abstract:Objective To investigate the suppression of cell proliferation of SGC-7901 gastric cancer cells by combination γ-tocotrienol(γ-T3) with 5-FU in vitro. Methods The effect of γ-tocotrienol (0, 5, 10, 20, 30, 40μmol/L) and 5-FU (0, 30, 50, 70, 90, 110μmol/L) on proliferation of gastric cancer cell lines for 48h was examined by Cell Counting Kit-8 (CCK-8). To determine if the combined effect of γ-tocotrienol (20 or 30μmol/L) and 5-FU (30μmol/L) is synergistic, we calculated the value of coefficient of drug interaction (CDI). The apoptosis assay were tested by flow cytometric analysis. Results The treatment with γ-tocotrienol or 5-FU alone induced a significant dose-responsive growth inhibition(P < 0.05), whereas combination treatment with these agents synergistically inhibited the growth of SGC-7901 cells(P < 0.05). The results of flow cytometric analysis showed that the treatment with γ-tocotrienol or 5-FU alone initate apoptosis (P < 0.05), and combination treatment had more significant effect in apoptosis than these agents alone (P < 0.05). Conclusion The finding indicates that γ-tocotrienol can potentiate the effects of antiproliferative and apoptosis-inducing in SGC-7901 cells treatment by 5-FU. |
| keywords:SGC-7901 γ-T3 5-FU Combination treatment |
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